Journal of Functional Foods：鱼油对酒精性肝损伤大鼠脑组织胰岛素抵抗的影响
文摘
2024-07-24 09:00
英国
臺北醫學大學--Suh-Ching Yang研究团队在Journal of Functional Foods发表题目为“Effects of fish oil on insulin resistance in the brains of rats with alcoholic liver damage”研究论文（鱼油对酒精性肝损伤大鼠脑组织胰岛素抵抗的影响）。摘要： 本研究旨在探讨鱼油对大鼠酒精性脑损伤的影响，重点研究其对胰岛素信号通路的影响。36只雄性Wistar大鼠分为对照组（Lieber-DeCarli控制液体饮食）、CF25组（控制饮食+25%鱼油替代品）、CF57组（控制饮食+57%鱼油替代品）、E组（Lieber-DeCarli乙醇液体饮食）、EF25组（乙醇饮食+25%鱼油替代品）、EF57组（乙醇饮食+57%鱼油替代品）。还进行了行为学测试。8周后，E组表现出脑损伤，包括核浓缩锥体细胞和离子化钙结合衔接分子1（Iba-1）抗体阳性。E组海马胰岛素受体底物-1（IRS-1）和下丘脑糖原合酶激酶3β（GSK3β）水平较高，而肝脏IRS-1、海马胰岛素样生长因子-1（IGF-1）受体和葡萄糖转运蛋白4（GLUT4）水平较低。鱼油替代后，脑核凝聚减弱，海马IRS-1和下丘脑GSK3β显著降低。研究结果：2.2. 肝损伤2.2.2. 肝组织病理学检查Fig. 1. Effects of fish oil on liver histopathological changes and hepatic CYP2E1 protein expression in rats with chronic ethanol feeding.2.3. 脑损伤评估及行为测试2.3.1. 脑组织病理学检查2.3.2. 海马炎症反应Fig. 2. (A) Histopathological changes of hippocampus in rats with chronic ethanol feeding. (B) Immunohistochemical (IHC) staining with anti-GFAP antibody of the hippocampus in chronic ethanol-fed rats. (C) IHC staining with anti-Iba1 antibody of the hippocampus in chronic ethanol-fed rats.2.3.3. Y型迷宫测试2.3.4. NOR2.3.5. OFTFig. 3. Effects of fish oil on behavior tests performance in rats with chronic ethanol feeding.2.4. IR2.4.1. 血清IGF-1浓度2.4.2. 肝脏胰岛素信号蛋白表达Fig. 4. (A) Effects of fish oil on the regulating factors of glucose metabolism in rats with chronic ethanol feeding. (B-I) Effects of fish oil on hepatic insulin metabolism-related protein expression in chronic ethanol-fed rats.2.4.3. 海马中胰岛素信号蛋白的表达Fig. 5. Effects of fish oil on hippocampal insulin metabolism-related protein expression in rats with chronic ethanol feeding.2.4.4. 下丘脑胰岛素信号蛋白的表达Fig. 6. Effects of fish oil on hypothalamic insulin metabolism-related protein expression in rats with chronic ethanol feeding.原文链接：https://doi.org/10.1016/j.jff.2024.106369
