天津科技大学食品学院杨瑞教授团队在Food Hydrocolloids发表题目为“Fabrication of the ferritin-mannoprotein shell-core heteroprotein complex for stabilization of bioactive molecules”研究论文（制造铁蛋白-甘露糖蛋白壳核异蛋白复合物以稳定生物活性分子）。摘要： 铁蛋白具有笼状结构，腔体直径为8nm，能够包裹生物活性分子；甘露糖蛋白是一种高度糖基化的蛋白质，具有N-糖基化或O-糖基化结构；这两种蛋白质由于性质不同，有可能形成异质蛋白复合物。本研究旨在制备甘露糖蛋白-铁蛋白异蛋白壳核复合物（MFH），研究铁蛋白与甘露糖蛋白之间的相互作用，并评估MFH对EGCG分子的结合行为、结构、稳定性和保护作用。ζ电位测量表明，MFH的形成主要是由两个带相反电荷的蛋白质之间的静电相互作用驱动的。进一步利用铁蛋白的自组装特性，制备出笼状铁蛋白包覆甘露糖蛋白-铁蛋白异蛋白复合物（EMFH），包覆率为18.8%（w/w）。EMFH呈规则球形聚集，粒径增大，原位相对湿度约为257.2±5.8nm。此外，与笼状铁蛋白（EF）相比，EMFH提高了EGCG的热稳定性和光稳定性，并有利于EGCG在模拟胃肠消化过程中的控制释放。本研究拓展了基于笼状铁蛋白和甘露糖蛋白的异蛋白复合物在稳定生物活性化合物中的应用。研究结果：3.1 铁蛋白的制备Fig. 1. (a) Image for the shell-like ferritin; the outer and inner diameters were 12 and 8 nm. (b) SDS-PAGE analysis of ferritin in lane 1.3.2 ζ电位分析Fig. 2. (a) The ζ -potentials of ferritin and mannoprotein with the pH variations. (b) The ζ -potentials of mannoprotein-ferritin heteroprotein complexes (MFH) with different mixing mass ratios of ferritin/mannoprotein from 4:1 to 1:4.3.3 浊度分析Fig. 3. The turbidity curves of single ferritin and mannoprotein, and the mannoprotein-ferritin heteroprotein complexes (MFH) with different mixing mass ratios of ferritin/mannoprotein from 4:1 to 1:4.3.4 铁蛋白-甘露糖蛋白异蛋白的尺寸分布Fig. 4. DLS of the different samples.3.5 MFH二级结构分析Fig. 5. (a) CD spectra (290-260 nm) of the ferritin, mannoprotein, and the mannoprotein-ferritin heteroprotein complexes (MFH) with a mixing mass ratio of 2:1 (mannoprotein/ferritin). (b) CD spectra (260–320 nm) of the ferritin, mannoprotein, and the MFH with a mixing mass ratio of 2:1 (mannoprotein/ferritin).3.7. 荧光分析Fig. 6. Fluorescence spectrum of ferritin, mannoprotein, EGCG, EGCG-encapsulated ferritin (EF), mannoprotein-ferritin heteroprotein complexes (MFH), and EGCG-encapsulated mannoprotein-ferritin heteroprotein complexes (EMFH)3.8. EMFH 的 TEM 和 DLS 分析Fig. 7. (a) TEM image of ferritin. (b) TEM image of EGCG-encapsulated ferritin complexes (EF). (c) TEM image of mannoprotein-ferritin heteroprotein complexes (MFH). (d) TEM image of EGCG-encapsulated mannoprotein-ferritin heteroprotein complexes (EMFH).3.10. EMFH在模拟胃肠消化过程中EGCG释放分析Fig. 8. The release rate of the EGCG in EGCG-encapsulated ferritin (EF), EGCG-encapsulated mannoprotein-ferritin heteroprotein complexes (EMFH), and EGCG-mannoprotein-ferritin mixture (EMFM) in simulated gastric/intestinal tracts over 2 h of incubation. (a) The simulated pepsin tract. (b) The simulated trypsin tract.原文链接：https://doi.org/10.1016/j.foodhyd.2024.110489
