济南大学生物学院冯润良副教授团队在Food Hydrocolloids发表题目为“pH-responsive hydrogel containing curcumin-loaded lipid nanocapsules for oral curcumin’s stability, bioaccessibility and intestinal absorption improvement”研究论文（含有姜黄素脂质纳米胶囊的 pH 响应性水凝胶，用于改善口服姜黄素的稳定性、生物可及性和肠道吸收）。摘要：具有合适稠度和缓释特性的液体配方可以提高吞咽困难患者的依从性。在研究中，我们制备了载姜黄素的脂质纳米胶囊 (Cur-LNCs)，并将其加入海藻酸钠、糊精、氯化钙和柠檬酸钠的混合物中，形成 pH 响应性预水凝胶溶液 (SA-DEX:Cur-LNCs)。SA-DEX:Cur-LNCs 表现出 pH 响应性溶液-凝胶转化：在接近中性（pH = 7）时溶解，在胃酸（pH = 2.5）下迅速凝胶化为强弹性凝胶。SA-DEX:Cur-LNCs 可有效提高 Cur 的热稳定性和 pH 稳定性，并保持其抗氧化作用。有趣的是，与游离 Cur 相比，SA-DEX:Cur-LNCs 在胃肠道中的生物可及性显著提高至 37.00%。SA-DEX:Cur-LNC 还显著改善了 Cur 的小肠吸收，比游离 Cur 高 16 倍。因此，在 pH 响应性预水凝胶中加入脂质纳米胶囊为疏水性生物活性化合物提供了一种新的口服给药平台，可改善吞咽困难患者的依从性。研究结果：3.1. Cur-LNC 的制备Fig. 1. (A) Preparation and structure of Cur-LNCs and SA-DEX:Cur-LNCs; (B) Aqueous solution of Cur (left) and Cur-LNCs (right) (800 μg/mL); (C) Photographs of SA-DEX:Cur-LNCs before (left) and after (right) simulated gastric acid treatment; (D) Photographs of SA:Cur-LNCs before (left) and after (right) simulated gastric acid treatment.3.2 SA:Cur-LNCs 和 SA-DEX:Cur-LNCs 的制备和表征Fig. 2. (A) Strain sweep of the SA:Cur-LNCs in shear rheology at a frequency of 1 Hz; (B) Frequency sweep of SA:Cur-LNCs and SA-DEX:Cur-LNCs; (C) FT-IR spectra of free Cur, blank-LNCs, Cur-LNCs, SA-DEX:blank-LNCs and SA-DEX:Cur-LNCs; (D) In vitro release curves of free Cur, Cur-LNCs and SA-DEX:Cur-LNCs at 37 °C (n = 4).3.4 Cur-LNCs 和 SA-DEX:Cur-LNCs 对 Cur 稳定性的影响Fig. 3. (A) Thermal stabilities of free Cur, Cur-LNCs and SA-DEX:Cur-LNCs in PBS solution (pH 7.0) at different temperatures for 10 min (n = 3, *p < 0.05); (B) Stabilities of free Cur, Cur-LNCs and SA-DEX:Cur-LNCs in PBS solution of different pH at room temperature (n = 3, *p < 0.05).3.5. Cur-LNCs 和 SA-DEX:Cur-LNCs 的抗氧化活性Fig. 4. (A) DPPH• scavenging of free Cur, Cur-LNCs and SA-DEX:Cur-LNCs (n = 3, *p < 0.05); (B) ABTS•+ scavenging of free Cur, Cur-LNCs and SA-DEX:Cur-LNCs (n = 3); (C) Fraction of Cur released from different formulations during incubation in stimulated gastric and small intestinal fluids: free Cur, Cur-LNCs and SA-DEX:Cur-LNCs (n = 4, *p < 0.05); (D) Absorption of free Cur, Cur-LNCs and SA-DEX:Cur-LNCs by everted rat intestinal sacs (n = 4, *p < 0.05).原文链接：https://doi.org/10.1016/j.foodhyd.2024.110488
