西北农林科技大学食品科学与工程学院王海华副教授团队在Food Packaging and Shelf Life发表题目为“Development of multifunctional zein-based films engineered with gallic acid and Ag NPs loaded γ-CD-MOFs for pork preservation”研究论文（开发以没食子酸和载银纳米粒子的 γ-CD-MOF 为载体的多功能玉米醇溶蛋白薄膜用于猪肉保鲜）。摘要： 人们对食品质量和消费者安全的日益重视促使研究人员探索包装材料的绿色制造技术。在这项研究中，γ-环糊精金属有机骨架 (γ-CD-MOF) 被制备为没食子酸 (GA) 和银纳米粒子 (Ag NPs) 的有前途的纳米载体。这些纳米载体 (GA-Ag@CD-MOF) 被进一步掺入玉米醇溶蛋白基质中，以制造多功能复合膜，称为 MOFs-Zein。扫描电子显微镜（SEM）和原子力显微镜（AFM）分析表明制备的复合膜具有良好的生物相容性，GA-Ag@CD-MOFs均匀分散在膜基质中。傅里叶变换红外光谱（FT-IR）结果表明，GA-Ag@CD-MOFs的加入有利于与基质聚合物形成分子间氢键。GA-Ag@CD-MOFs的加入使复合膜的拉伸强度提高了19.70%，防水性能提高了57.76%，紫外线防护效果几乎达到100%。γ-CD-MOFs对GA的优异控释能力使复合膜在储存100天后仍能保持其强大的初始抗氧化能力。此外，复合膜对大肠杆菌 O157:H7 和肠道沙门氏菌具有高效抗菌活性。急性毒理学研究表明，在 GA-Ag@CD-MOF 处理的小鼠中未发现与组织病理学相关的毒性。与对照膜相比，当应用于新鲜猪肉时，复合膜显著降低了生肉的关键质量指标。总体而言，这项工作提出了一种有前途的方法来制造复合膜，作为绿色功能性食品包装的可持续和功能性解决方案。研究结果：3.1 GA-Ag@CD-MOFs的表征Fig. 1. SEM micrograph (a) of γ-CD-MOFs, Ag@CD-MOFs and GA-Ag@CD-MOFs; TEM micrograph (b) of GA-Ag@CD-MOFs; FT-IR spectra (c), XRD patterns (d), TGA thermograms (e) and DTG curves (f) of GA, γ-CD-MOFs, Ag@CD-MOFs and GA-Ag@CD-MOFs; N2 adsorption patterns (g) and pore size distributions (h) of γ-CD-MOFs, Ag@CD-MOFs and GA-Ag@CD-MOFs.3.2 薄膜的表征Fig. 2. SEM (a) of the surface and cross-sectional morphology; AFM images (b) of 2D and 3D microstructures; FT-IR spectra (c) of the pure zein film and MOFs-Zein films; XRD patterns (d) of the pure zein film, GA-Ag@CD-MOFs and MOFs-Zein films.3.3 表面颜色和光学特性Fig. 3. Photographs (a), UV–vis transmittance spectra (b), the thickness (c), TS and EB (d), MC (e), WS (f), and WVP (g) of the pure zein film and MOFs-Zein films.3.7. 释放研究Fig. 4. Release of GA from MOFs-GA and MOFs-Zein-8 in DI water (a); Ag+ migration of MOFs-Zein-8 in DI water (b); antioxidant activities of Zein-GA film and MOFs-Zein films determined by DPPH (c) and ABTS (d) assay.3.9. 抗菌活性Fig. 5. The growth curves and SEM microscopic images of GA-Ag@CD-MOFs against E. coli O157:H7 (a and b) and S. enterica (c and d); the survival rates and photographs of bacterial colonies of the pure zein film and MOFs-Zein films against E. coli O157:H7 (e and f) and S. enterica (g and h).3.10. 安全性评价Fig. 6. Caco-2 cell viability (a) and relative hemolysis rates (b) at different concentrations of Ag@CD-MOFs and GA-Ag@CD-MOFs; Photomicrographs of histological sections of 14 day acute oral toxicity study on liver (10 ×), spleen (10 ×), lung (10 ×), kidney (10 ×), and colon (20 ×) (c); Effects of different films on visual appearance (d), pH (e), TVC (f), TVB-N (g), and TBARs (h) of pork during refrigerated storage (4 ℃) for 12 day.原文链接：https://doi.org/10.1016/j.fpsl.2024.101352
