浙江大学医学院李兰娟院士团队在Food & Function发表题目为“Synergistic effects of Ligilactobacillus salivarius Li01 and psyllium husk prevent mice from developing loperamide-induced constipation”研究论文（唾液乳杆菌 Li01 与车前草壳的协同作用可防止小鼠发生洛哌丁胺引起的便秘）。摘要： 便秘是一种胃肠道疾病，其特征是排便困难、腹痛和腹胀，严重影响身心健康。Ligilactobacillus salivarius Li01 (Li01) 是一种已知可以预防小鼠便秘的益生菌，而洋车前子壳 (PSH) 是一种具有高保水性的膳食纤维，可作为肠道润滑剂。本研究调查了 Li01 和 PSH 联合治疗对洛哌丁胺诱发便秘的小鼠的影响。联合治疗比单独使用 Li01 或 PSH 更有效，可以改善胃肠道运输速度、增加粪便含水量并调节血清胃肠道激素浓度。这些有益作用与胃肠道中丁酸水平较高和非 12-OH 胆汁酸 (BA) 比例较高有关。这些保护作用不受肠道微生物群变化的影响。此外，Li01 体外产生丁酸并发酵 PSH。我们的研究结果表明，益生菌 Li01 和益生元 PSH 可协同预防小鼠便秘，凸显了它们作为功能性食品成分的潜力。研究结果：3.1. Li01 和 PSH 治疗可防止小鼠便秘Fig. 1 The effects of treatment with Li01 and psyllium husk (PSH) were investigated in mice with loperamide-induced constipation.3.2 Li01和PSH对结肠形态和肠道菌群组成的影响Fig. 2 Colonic morphology and composition of the gut microbiota were evaluated in mice in the different treatment groups.3.3 Li01和PSH对肠道菌群代谢的影响Fig. 3 Cecal concentrations of short-chain fatty acids (SCFAs) (A–G) and colon concentrations of total bile acids (BAs) (H), secondary BAs (I), non-12-OH BAs through 12-OH BAs (J), hyodeoxycholic acid (HDCA) (K), murideoxycholic acid (MDCA) (L) were measured and evaluated in mice in the different treatment groups.3.4 肠道菌群破坏对 Li01 和 PSH 保护作用的影响Fig. 4 The effects of treatment with Li01 and PSH were investigated in mice with loperamide-induced constipation and with their microbiota disrupted by antibiotic treatment.3.5. 肠道菌群紊乱的便秘小鼠接受 Li01 和 PSH 治疗后代谢相关变化Fig. 5 Concentrations of SCFAs and BAs were measured in feces of mice gavaged with loperamide and with their microbiota disrupted by antibiotic exposure.3.6. Li01代谢组学特征的体外表征Fig. 6 Metabolomic features and characteristics of Li01 growing in vitro were determined.原文链接：DOI https://doi.org/10.1039/D4FO04444D
