内容简介
本研究论文聚焦一种用于工程化生物组织构建的含氧胶体生物墨水。确保充足的氧气供应对于生物打印应用的成功至关重要，因为它促进了组织融合和自然再生。为保证目标细胞的存活，需要在3D打印结构中产生不同组织的氧气浓度变化，并精确再现特定组织的氧气水平。尽管释放氧气的生物材料（如释氧微粒）表现出增强受伤组织微环境氧气供应的前景，但这种方法是否可扩展到大尺寸组织，以及不同释氧微粒浓度的组织特异性生物墨水是否可以用于3D打印仍然未知。本研究通过引入一类创新的含氧生物墨水，将胶体基微凝胶与释氧微粒相结合，来填补这一关键空白。本研究将纳米级过氧化钙（nCaO2）和氧化锰纳米片（nMnO2）引入疏水性聚合物微粒中，使氧气释放得以精确调节，同时控制过氧化氢的释放。此外，利用水相两相体系可实现含氧和细胞相容性胶体凝胶的制备。本研究全面评估了所得到的生物墨水的基本特性，包括其流变行为、可打印性、形状保真度、力学性能和氧气释放特性。此外，本研究通过细胞负载氧化胶体生物墨水制备的印刷构建物展示了宏观可扩展性和细胞相容性。通过展示基于挤出的生物打印的效果，本研究强调了它可用于制造仿生组织的能力，表明它在新应用领域的潜力。这些研究成果通过实现高细胞存活率和能够模拟特定供氧组织的可扩展性，推动了生物打印领域的发展。因此，这项工作为增强生理相关性的功能组织的发展提供了一条有希望的途径。
引用本文（点击最下方阅读原文可下载PDF）
Jeong SH, Hiemstra J, Blokzijl PV, et al., 2024. An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs. Bio-des Manuf 7(3):240–261. https://doi.org/10.1007/s42242-024-00281-7
文章导读
图1 图解了胶体凝胶、释氧微粒和生成氧气的胶体生物墨水的制备过程以及它们在组织构建制备中的潜在应用
图2 不同GelMA:明胶比例下混合胶凝胶的特性鉴定
图3 释氧微粒（包括nCaO2和nMnO2纳米颗粒）的特征
图4 释氧微粒（OMP）载体胶体生物墨水的表面形态及OMP-Gels的后交联水解稳定性、酶稳定性和氧释放行为的表征
图5 OMP的细胞毒性和载细胞OMP-Gel打印结构的细胞存活率
参考文献
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