内容简介
本研究论文聚焦具有微米空间分辨率的氧气感测的高度敏感比率荧光纤维矩阵。氧（O2）感应矩阵是在长期细胞培养中实时监测细胞外O2消耗水平的有前景的工具。在本研究中，研究人员利用静电纺丝法制备了比例氧感应膜，该方法是一种简便、低成本、可扩展和可靠的纳米纤维制造方法。将聚己内酯和聚二甲基硅氧烷聚合物与二氯化三(4,7-二苯基-1,10-邻菲罗啉)钌(II) 和异硫氰酸罗丹明B混合，其中前者用作氧感应探针，后者用作参考染剂。利用扫描电子显微镜对功能化支架进行形态学表征，并通过傅里叶变换红外光谱、热重分析和水接触角测量获得其物理化学特性。利用共聚焦激光扫描显微镜对感应能力进行研究，包括光漂白、可逆性和校准曲线等，以检测不同溶解氧（DO）浓度。电纺感应纳米纤维对生理-病理范围内0.5%至20%溶解氧浓度的变化具有高响应能力，并且在比例成像下具有良好的稳定性。此外，这些感应系统对于促进三种癌细胞系（即转移性黑色素瘤细胞系SK-MEL2、乳腺癌细胞系MCF-7和胰腺导管腺癌细胞系Panc-1）的细胞生长和黏附具有高生物相容性，在体外再现了适宜的生物环境。这些氧感应生物材料有潜力在药物测试/验证和组织再生过程中测量由周围氧含量变化引起的细胞代谢变化。
引用本文（点击最下方阅读原文可下载PDF）
Grasso G, Onesto V, Forciniti S, et al., 2024. Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution. Bio-des Manuf 7(3):292–306. https://doi.org/10.1007/s42242-024-00277-3
文章导读
图1 比例式O2传感光纤的微观形态
图2 比例氧传感纤维的结构表征
图3 CLSM成像和比例氧传感纤维的传感性能
图4 生物相容性和比例氧感纤维上的细胞增殖
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