内容简介
本研究论文聚焦用于组织工程的3D打印电导性pHEMA-co-MAA纳米粒子水凝胶。组织工程（TE）作为一种潜在的解决方案，被广泛探索以满足用于替换病变组织和组织再生的临床需求。在本研究中，我们开发了基于聚(2-羟乙基甲基丙烯酸酯-共-甲基丙烯酸) (pHEMA-co-MAA) 的水凝胶，搭载了新合成的导电聚(3,4-乙二醇二噻吩) (PEDOT) 和聚吡咯 (PPy) 纳米颗粒（NPs），并通过三维（3D）打印将这些水凝胶制成组织工程构件。NPs在墨水中的存在至关重要，因为它们改变了打印过程中的流变性质。所有样品都表现出适当的剪切稀化特性，从而为3D打印窗口的优化提供了可能。样品被3D打印成预先确定的圆盘状构型，其直径为2 mm，高度为10 mm。我们观察到，NPs破坏了凝胶交联效率，导致样品具有较短的降解时间和在450至550 kPa范围内的压缩力学性能。打印水凝胶的导电性随着NP浓度的增加而增加至(5.10±0.37)×10−7 S/cm。与皮质星形胶质细胞培养的体外研究表明，暴露于pHEMA-co-MAA NP水凝胶中可获得较高的细胞存活率和增殖率。最后，与表皮葡萄球菌的水凝胶抗菌研究表明，开发的水凝胶对细菌生长产生了影响。综上，这些材料在各种组织工程策略中都显示出潜力。
引用本文（点击最下方阅读原文可下载PDF）
De Nitto S, Serafin A, Karadimou A, et al., 2024. Development and characterization of 3D-printed electroconductive pHEMA-co-MAA NP-laden hydrogels for tissue engineering. Bio-des Manuf 7(3):262–276. https://doi.org/10.1007/s42242-024-00272-8
文章导读
图1 纳米颗粒合成示意图
图2 pHEMA-co-MAA纳米颗粒水凝胶的体外间接接触实验的示意图
表1 3D打印的pHEMA-co-MAA纳米颗粒样品，控制组和所有带有纳米颗粒的样品每层曝光时间分别为25秒和70秒
图3 在使用pHEMA-co-MAANP水凝胶处理的培养基中培养时，细胞形态学在体外呈现出较佳的活性星形胶质细胞
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Bio-Design and Manufacturing（中文名《生物设计与制造》），简称BDM，是浙江大学主办的专业英文双月刊，主编杨华勇院士、崔占峰院士，2018年新创，2019年被SCI-E等库检索，2023年起改为双月刊，2023年公布的最新影响因子为7.9，位列JCR的Q1区，14/96，年末升入《2023年中国科学院文献情报中心期刊分区表》医学一区。
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