内容简介
本研究论文聚焦用于动态评估心肌细胞膜再愈合的集成电穿孔和纳米多孔传感电极阵列。由于细胞-电极界面松散，传统的平面微电极阵列（MEA）在记录细胞内动作电位方面存在不足。为了高灵敏地研究细胞内电生理信号，使用电穿孔来获得细胞内记录。本研究建立了一种基于纳米多孔电极阵列（NPEA）的生物传感系统，该系统集成了电穿孔和信号采集，可以在很长一段时间内动态地、灵敏地记录心肌细胞的胞内电位。此外，纳米多孔电极可以诱导细胞膜的突出，增强细胞-电极界面耦合，从而促进有效的电穿孔。整个记录过程中的电生理信号可以根据信号变化进行定量分段分析，从而等效地反映电穿孔心肌细胞膜的动态演化。我们认为，本研究提出的低成本、高性能的纳米多孔生物传感平台可以动态记录细胞内动作电位，评估心肌细胞电穿孔，从而为研究心脏病药理学提供新的策略。
引用本文（点击最下方阅读原文可下载PDF）
Sheng W, Li Y, Qin C, et al., 2024. Integrated nanoporous electroporation and sensing electrode array for total dynamic time-domain cardiomyocyte membrane resealing assessment. Bio-des Manuf (Early Access). https://doi.org/10.1007/s42242-024-00308-z
文章导读
图1 基于纳米多孔电极阵列的传感器件用于动态评估心肌细胞膜再愈合的示意图
图2 三维纳米多孔电极阵列的制备过程与表征
图3 通过持续电生理信号记录来动态评估电穿孔心肌细胞膜的再愈合
图4 不同形态电极阵列用于电生理信号记录的比较
图5 不同孔径的三维纳米多孔电极阵列用于电生理信号记录的比较
参考文献
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