内容简介
本研究论文聚焦静电纺丝/3D打印一体化多孔支架引导比格犬口腔组织再生。膜与骨填充移植物的联合应用是引导口腔软硬组织再生和功能重建的经典治疗方法。然而，由于骨粉易移位且机械支撑力不足，膜与骨粉联合应用不适用于皮质骨支撑不足或骨缺损较大的病例。GTR/GBR膜与三维多孔支架结合可能为大尺寸软硬组织缺损的修复和功能重建提供有效的解决方案。本研究将聚乳酸-羟基乙酸（PLGA）/鱼胶原（FC）电纺丝膜（PFC）与3D打印的PLGA/纳米羟基磷灰石（HA）支架（PHA）结合，成功制备了具有梯度结构的可生物降解多孔支架。上层纤维膜与下层3D支架主成分均包含PLGA，其成分的一致性使膜与3D支架之间形成牢固的界面结合。体外细胞实验显示PFC膜（上层）可有效阻止L929细胞向3D打印骨修复支架迁移。比格犬口腔软硬组织缺损修复实验显示该复合梯度支架能有效引导口腔缺损组织的再生。这些研究结果表明设计制备的梯度支架在促进口腔大尺寸软硬组织再生和功能重建方面具有巨大潜力。
引用本文（点击最下方阅读原文可下载PDF）
Yuan L, Yuan C, Wei J, et al., 2024. Electrospinning/3D printing-integrated porous scaffold guides oral tissue regeneration in beagles. Bio-des Manuf (Early Access). https://doi.org/10.1007/s42242-024-00311-4
文章导读
图1 一体化支架构建及动物实验流程图
图2 一体化支架结构形貌
图3 一体化支架的细胞相容性及电纺膜阻止成纤维细胞向3D打印支架迁移
图4 Micro-CT分析支架植入4和8周后拔牙窝新骨再生
图5 支架植入后8周比格犬牙槽骨标本的组织学分析
参考文献
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