内容简介
本综述论文聚焦细胞之间的相互作用在3D体外微生理学疾病模型中的体细胞布局菌群-肠-脑轴上的影响。微生物群-肠道-脑轴(MGBA)已经成为大脑和肠道这两个主要器官系统之间双向沟通的关键前景。这两个器官系统之间的稳态使身体能够正常运作,而微生物失调已经有长期的疾病病因学证据。最常见的通信路径是微生物释放代谢物、可溶性神经递质和免疫细胞。然而,每条路径都与复杂的路径相互交织。随着体外模型的出现以及三维(3D)培养和跨膜技术的普及,工程化已经更容易地用于科学理解神经退行性疾病。本文简要回顾了肠道微生物群与大脑之间可能的通信路径,进一步阐述了三种主要疾病:自闭症谱系障碍、帕金森病和阿尔茨海默病,这些疾病在儿童和老年人中都很常见。这些疾病也会降低患者的生活质量。因此,借助体外模型的当前进展来更深入地理解这些疾病是至关重要的。实验室中对MGBA的重建使用了许多先进的分子技术和生物材料。球体和器官样结构与单层细胞相比提供了更逼真的细胞和组织结构图像。将它们与跨膜系统相结合,在允许它们之间传递物理和化学信号的同时可以隔离两个系统(上/下流道)。生物打印和微流控芯片等尖端技术提供了动态性,可能是体外模型的未来方向。
引用本文(点击最下方阅读原文可下载PDF)
Alam K, Nair L, Mukherjee S, et al., 2024. Cellular interplay to 3D in vitro microphysiological disease model: cell patterning microbiota–gut–brain axis. Bio-des Manuf 7(3):320–357. https://doi.org/10.1007/s42242-024-00282-6
文章导读
图1 菌群-肠-脑轴相关系统和通路
图2 菌群-肠-脑轴与神经退行性疾病
图3 细胞团与类器官神经元模型
图4 肠道上皮屏障的假设发展
图5 3D渗透模型机制和细胞相互作用
图6 菌群-肠-脑轴的3D体外建模,使用Transwell和微流控系统
参考文献
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