作者
刘雨行a,1, 刘志强b,1, 李健b, 闫晓敏a, 许炜迪a, 易乐a, 涂长春a,c，何彪a, *
单位
a 中国农业科学院，长春兽医研究所，病原与生物安全全国重点实验室，吉林省人兽共患病防控重点实验室b 双河省级野生动物疫病监测站
c 扬州大学，江苏重要动物传染病和人兽共患病防控协同创新中心
摘要
快速诊断对于新发传染病的防控至关重要。本研究使用纳米孔测序对一例狐狸死亡病例进行快速病毒组分析，结果使用6个小时便完成从样品接收到锁定病原。通过荧光定量RT-PCR和FAT检测，进一步验证了纳米孔测序诊断的准确性，并确认引起该狐狸死亡的原因是类北极型狂犬病病毒的感染。该研究展示了纳米孔测序在新发传染病快速诊断中的应用潜力，同时也表明未来需要解决测序准确性的问题。
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Fig. 1. Nanopore sequencing-based diagnosis of a fox’s death case. A Workflow of nanopore sequencing-based diagnosis and the time consumption from sample receipt to the detection of the first RABV read.
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Fig. 1. B Read counts at the 5 timepoints of sequencing. C Read classification at the 5 timepoints of sequencing. ![]()
Fig. 1. D Detection of RABV in the positive and negative controls and the clinical brain sample using FAT. E RABV viral loads assessed using genomic copy in these organs. ![]()
Fig. 1. F Distribution of the three nanopore RABV-like reads against the nearly complete genome sequenced using Illumina platform and RT-PCR. Fig. 1. G Phylogenetic analysis of strain XKVv01 based on Read 1 and its counterparts. H Phylogenetic analysis of strain XKVv01 based on the overlaps of Reads 2 and 3 and their counterparts. Fig. 1. I Overview of the variations of the three reads compared to the Illumina sequence.相关阅读：
《中国病毒学（英文）》Virologica Sinica, 是中国科学院武汉病毒研究所和中国微生物学会共同主办的病毒学领域的专业学术期刊。主要刊载病毒学及分支领域的前沿研究及最新进展。本刊最新影响因子（JCR2023）4.3, 5年影响因子 4.3, 排位进入病毒学领域Q1区，CiteScore 2023 9.3, 连续十一年入选“中国最具国际影响力学术期刊”（TOP 5%）。期刊于2022年变更为以金色开放获取模式出版的开源期刊（Open Access Journal），与科爱出版社合作全球出版传播。本刊为中国科技核心期刊，且被SCI、PubMed/Medline、PubMed Central、Scopus等数据库收录。
