CNS 2024 | 专题研讨会：先进技术

报告人

Tomas Cizmar

Leibniz Institute of Photonic Technology

Our team develops hair-thin endoscopes with the aim of acquiring sub-micron resolution images anywhere in the living brain. Unlike a ‘classic’ imaging system relying on optical fields from one plain to another, this technology exploits holographic control of light propagating through optically complex multimode optical waveguides. The introductory lecture will explain the principles in an accessible form and present the most significant achievements of this prospect so far (up-to-date at the time point of presentation, including unpublished data), together with the most significant challenges to be addressed.

俞婷婷

华中科技大学

华中科技大学武汉光电国家研究中心副研究员，博士生导师。研究方向为组织光学成像新技术新方法，具体包括组织光透明成像原理与方法、神经光学成像应用。一直致力于构建基于光透明的组织光学成像方法，应用于中枢神经系统、周围神经系统与其他生物组织器官三维整体结构信息的获取与重建。在Science Advances, Advanced Science, Theranostics，Journal of Biophotonics等期刊发表SCI论文20余篇，授权发明专利1项。

孔令杰

清华大学

孔令杰 副教授、博导。2016年入选国家海外高层次人才引进计划（青年项目）。2012年在清华大学获得工学博士学位，随后在哈佛大学、霍华德·休斯医学研究所Janelia研究校园、普渡大学从事博士后研究，2017年加入清华大学精密仪器系。

贾宏博

中国科学院苏州生物医学工程技术研究所

贾宏博，博士，研究员，博士生导师，主要从事双光子显微成像技术研发和脑神经元信号解析的研究工作。

刘艳颜

复旦大学

刘艳颜，复旦大学青年研究员。研究方向：

材料生物学（材料物理与化学，材料学）

1.新型光/电/磁功能材料的合成方法学研究;

2.新型功能材料用于神经科学研究（神经显像与神经调控）。

课题组的研究方向为无机生物化学与医用功能材料，是材料学、化学、物理学、生物学、医学等多学科综合交叉领域的前沿研究。

近年来发表论文：

1.Yanyan Liu, Yaqin Jiang, Meng Zhang, Zhongmin Tang, Mingyuan He, Wenbo Bu*.Modulating Hypoxia via Nanomaterials Chemistry for Efficient Treatment to Solid Tumors.Accounts of Chemical Research,2018, 51(10), 2502-2511. (Invited Review).

2.Yanyan Liu, Xianfu Meng,Wenbo Bu*.Upconversion-based Photodynamic Cancer Therapy. Coordination Chemistry Reviews, 2019, 379, 82-98. (Invited Review).

3.Yanyan Liu, Meng Zhang, Wenbo Bu*. Bioactive nanomaterials for ion‐interference therapy. VIEW. 2020;1:e18. (Invited Review).

报告人

Ryohei Yasuda

Max Planck Florida Institute

We have designed tools that enable us to image signaling activity at the level of individual synapses and to perturb signaling processes using optogenetic approaches. These techniques have provided unprecedented insights into the mechanisms that underlie synaptic plasticity, circuit function, and behavioral adaptations.

1. Majumder et al. (2023). Cell-type-specific plasticity shapes neocortical dynamics for motor learning. BioRxiv, 2023.08.09.552699.

2. Jain et al. (2023). Dendritic, delayed, and stochastic CaMKII activation underlies behavioral time scale plasticity in CA1 synapses. BioRxiv, 2023.08.01.549180.

3. Espadas et al. (2023) A synaptically targeted lncRNA, facilitates the consolidation of contextual fear memory. Res Sq [Preprint]

Haruhiko Bito

University of Tokyo

1. Kim et al. (2023) Social transmission of valence-linked new knowledge without firsthand experience in mice. bioRxiv 2023.08.27.555038

2. Pagano et al. (2023) Arc controls alcohol cue relapse by a central amygdala mechanism. Molecular Psychiatry

Tianyi Mao

Vollum Institute at Oregon Health & Science University

俄勒冈健康与科学大学Vollum研究所PI， 教授。研究方向：在小鼠模型中开发并实施了尖端技术，包括现代解剖学、计算、遗传学、成像和功能通路映射，以检查控制此类神经元连接及其神经调节的原理。理解感觉-运动相交互和运动控制的信号通路机制，行为和疾病是如何改变和调节是这些信号通路的机制。毛博士目前的重点是研究丘脑-皮质-纹状体通路的连通性和功能以及该通路下阿片类药物调节。与此同时，为了实现这些目标，毛教授致力于开发基因编码的环磷酸腺苷成像技术，以研究单细胞和亚细胞分辨率下活体组织对阿片类药物的细胞反应。1. Oostrom et al. (2023). Fine-tuning TrailMap: The utility of transfer learning to improve the performance of deep learning in axon segmentation of light-sheet microscopy images. Biorxiv 2. Ma et al. (2022). Locomotion activates PKA through dopamine and adenosine in striatal neurons. Nature

Jun Chu

Shenzhen Institute of Advanced Technology, CAS

cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular cAMP in intact organisms are largely unknown due to low sensitivity and/or brightness of current genetically encoded green and red fluorescent cAMP indicators. Here, we report the development of the new circularly permuted FP (cpFP)-based cAMP indicators, which exhibit a large fluorescence increase (a maximum ΔF/F0 of >1000% in HEK293T cells), decent brightness, appropriate affinity (a Kd of 0.5-3 μM) and fast response kinetics. We demonstrate that new cAMP sensors enable sensitive monitoring of endogenous cAMP signals in brain regions that are implicated in learning and motor control in living organisms such as fruit flies and mice.

1. Wang et al. (2022). A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging. Nature Communications

Jianan Qu

The Hong Kong University of Science and Technology

1. Yingzhu HE, et al., “Intravital microscopy of satellite cell dynamics and their interaction with myeloid cells during skeletal muscle regeneration", Science Advance, (2023)

2. Zhongya Qin, et al., “Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping”, Nature Biotechnology, (2022)

3. Wanjie Wu, et al., “Long-term in vivo imaging of mouse spinal cord through an optically cleared intervertebral window”, Nature Communications, V. 13, 1959 (2022)

4. Zhongya Qin, et al., "Adaptive optics two-photon endomicroscopy enables deep brain imaging at synaptic resolution over large volumes", Science Advances, 30 Sep 2020: Vol. 6, no. 40, eabc6521

5. Zhongya Qin, et al., "Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo", Nature – Light: Science & Applications, (2020)9:79

曾健智

深圳湾实验室

曾健智博士，2015年本科毕业于湖南大学生物学院，2021年毕业于北京大学前沿交叉学科研究院，获得神经科学博士学位，师从李毓龙教授，同年获得“吴瑞奖”。2022年加入深圳湾实验室从事博士后工作，合作导师为李毓龙教授（北大/深圳湾实验室）和薛天教授（中科大），主要研究方向：1）开发可基因编码的荧光探针，用于在活体上实时观测重要的信号分子的时空动态，如神经递质、荷尔蒙等；2）研究巴普洛夫学习范式中，信息整合时间窗口的神经调控机制。曾以第一作者身份在Cell 发表学术论文，其它参与的研究工作发表在Nature Biotechnology、Nature Methods 和Nature Neuroscience 等杂志。

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