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黄精多糖的理化性质、分子量和单糖组成分析
表1 黄精多糖的理化性质
图1 PSP的分离纯化及组成分析
黄精多糖的红外光谱分析
黄精多糖的核磁共振分析
图2 黄精多糖的一维和二维核磁共振波谱
表2 黄精多糖的1H和13C NMR化学位移
模拟唾液、胃液、小肠液消化黄精多糖
图3 模拟唾液、胃液、小肠液体外消化后PSP的还原糖含量、分子量的动态变化
模拟体外发酵黄精多糖
图4 人体粪便发酵48 h后的细菌生长曲线、pH和蛋白质含量变化
图5 体外发酵48 h后的总碳水化合物、还原糖和分子量的动态变化
黄精多糖对肠道菌群的影响
图6 PSP对人体肠道微生物在门、科和属水平上丰度的影响
短链脂肪酸（SCFAs）、吲哚含量分析
图7 黄精多糖组和对照组不同发酵时间点发酵液中总短链脂肪酸（A）、乙酸（B）、丙酸（C）、丁酸（D）、戊酸（E）、吲哚（F）含量变化
黄精多糖发酵液自由基清除能力分析
图8 黄精多糖发酵液的抗氧化能力分析
本研究从中药黄精中提取分离得到一种新型黄精多糖PSP，其分子量为3.06 kDa，主要由果糖、葡萄糖组成。该多糖主链包括α-D-Glcp-1→、→2-β-D-Fruf-1→两种糖苷键。PSP在上消化道能够保持结构稳定，在大肠中由肠道菌群分解代谢，产生大量有益于人体健康的短链脂肪酸，同时发酵后黄精多糖抗氧化能力提高。本论文为后续黄精多糖在生物医药和功能食品中的开发应用提供实验基础。
Structural characterization and prebiotic potential of polysaccharides from Polygonatum sibiricum
Zihan Qia,b,#, Tiexiang Gaob,#, Jingjing Lia, Shuhan Zhoua, Zhigang Zhanga, Mingzhu Yina, Haiming Hua,*, Hongtao Liua,*
a College of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China
b College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
# Both authors contributed equally.
*Corresponding author.
Abstract
Polygonatum sibiricum has been widely used due to its excellent biological activities. We prepared a novel polysaccharide from P. sibiricum (PSP) in this study. According a monosaccharide composition analysis, PSP was mainly composed of fructose and glucose with a molar percentage of 93.81:5.12. The main linkage types were identified as α-D-Glcp-1→ and →2-β-D-Fruf-1→. The molecular weight of PSP showed no significant change after simulated salivary and gastrointestinal digestion. However, PSP could be broken down by intestinal bacteria. Our findings revealed that PSP administration increased the abundance of probiotics such as Bifi dobacterium. Furthermore, the results showed that gut microbes could utilize PSP to produce short-chain fatty acids including acetic acid, propionic acid, and butyric acid. Also, the PSP fermentation broth displayed an excellent scavenging effect on free radicals, including 2, 2-diphenyl-1-picrylhydrazyl radical, superoxide radical, and hydroxyl radical. In summary, this study will help to promote the application of PSP as prebiotics in functional food and the medical industry.
QI Z H, GAO T X, LI J J, et al. Structural characterization and prebiotic potential of polysaccharides from Polygonatum sibiricum[J]. Food Science and Human Wellness, 2024, 13(4): 2208-2220. DOI:10.26599/FSHW.2022.9250184.
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