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Results and Discussion
图1 金银花多糖的分离纯化及结构表征
图2 金银花多糖对炎症相关转录因子（p-IκBα）和炎症蛋白质（iNOS和COX-2）表达的影响
图3 金银花多糖对斑马鱼巨噬细胞迁移的影响
图4 LJP-2的核磁共振光谱
Conclusion
刘宇，男，山东农业大学2020级硕士研究生，研究方向为农产品加工与贮藏工程，以第一作者发表SCI论文2篇，中文核心1篇。
郑振佳，山东农业大学食品科学与工程学院副教授，博士生导师。研究方向为果蔬加工、天然产物化学与农业标准化。山东省重点扶持区域引进急需紧缺人才，中国食品科学技术学会第三届青委会委员，山东省食品科学技术学会副秘书长，《食品工业科技》和Agriculture Communications青年编委。主持国家重点研发计划子课题等20项，主持研制阿卡波糖等6项国家标准，以第一或通讯作者在Journal of Agricultural and Food Chemistry等杂志发表论文60余篇，首位获中国商业联合会科学技术奖二等奖等4项。
Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships
Yu Liua,1, Hongjing Dongb,1, Dongxiao Sun-Waterhousec, Wenwen Lia, Bin Zhanga, Jinqian Yub, Zhichang Qiua,*, Zhenjia Zhenga,*
a Key Laboratory of Food Nutrition and Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
b Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
c School of Chemical Sciences, The University of Auckland, Private Bag 92019, New Zealand
1 Both authors contributed equally.
*Corresponding author.
Abstract
This study demonstrates the feasibility of producing three polysaccharides (neutral LJP -1, acidic LJP-2 and acidic LJP-3) with significant in vitro and in vivo anti-inflammatory activities from the flowers of Lonicera japonica. The three polysaccharides differed in chemical composition, molecular weight (Mw) distribution, glycosidic linkage pattern, functional groups and morphology. They exhibited excellent protective effects (in a dose-dependent manner) in lipopolysaccharide-injured RAW264.7 macrophages and CuSO4-damaged zebrafish via reducing NO production and inhibiting the overexpressions of inflammation-related transcription factors, inflammatory proteins and cytokines in the NF-κB/MAPK signaling pathways. Their anti-inflammatory effects varied owing to their different molecular characteristics and chemical compositions. Overall, LJP-2 at 400 μg/mL was the most effective. LJP-2 consisted mainly of →5)-α-L-Araf (1→, →4)-α-L-GalpA (1→ and →2)-α-L-Rhap (1→ residues with terminal T-β-D-Glcp. Thus, honeysuckle flowers are good sources of anti-inflammatory polysaccharides, and precise fractionation enables the production of potent anti-inflammatory agents for the development of functional foods and healthcare products.
LIU Y, DONG H J, SUN-WATERHOUSE D X, et al. Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships[J]. Food Science and Human Wellness, 2024, 13(4): 2197-2207. DOI:10.26599/FSHW.2022.9250183.
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