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RCE的化学成分表征
图1 色谱图和RCE中鉴定的化合物
RCE吸收入血成分鉴定
图2 RCE血清药物化学分析
RCE干预下的非靶向代谢组学研究
图3 RCE对结肠炎保护机制代谢组学研究的统计分析
RCE参与改变脂肪酸代谢和胆汁酸代谢预防结肠炎
本研究应用UHPLC-HR-MS技术阐明了RCE中的化学物质基础。结果鉴定出88个化合物，包括4个无环醇衍生物、12个苯乙烷衍生物、15个苯丙烷衍生物、5个苯甲酸衍生物、12种酚类和40种黄酮类化合物。其中，29种化合物在小鼠灌胃后可被吸收到血液中。共鉴定出8种代谢产物，由酪醇、咖啡酸和山奈酚原型经过羟基化、氧化、葡萄糖醛酸化或硫酸化过程生成。在结肠炎的病理生理条件下，观察到血浆类黄酮水平下降，酚类化合物硫酸化产物水平升高。代谢组学研究共鉴定出111种特征内源性代谢产物。MSEA结果显示结肠炎引起脂肪酸代谢、类固醇激素生物合成和胆汁酸代谢的改变。相应地，RCE可以通过改善脂肪酸代谢和次级胆汁酸代谢来预防结肠炎。本研究探讨了R. crenulata对结肠炎保护作用的药效物质基础和机制，为扩大其在结肠炎领域的应用奠定了基础。
彭瑜副教授/博士生导师，博士毕业于澳门大学，现就职于大连理工大学医学部，长期从事慢性病代谢调控及天然药物发现相关研究。目前，共发表SCI论文15篇（JCR一区论文9篇），其中第一作者或通讯作者论文10篇。主持国自然青年1项，博后面上基金1项，曾获江苏省卓越博士后计划。兼任Aging and Disease （Q1，IF=7.4）、Acta Materia Medica青年编委。
Reveal the pharmacodynamic substances and mechanism of an edible medicinal plant Rhodiola crenulate in DSS-induced colitis through plasma pharmacochemistry and metabolomics
Yu Penga, Xiaoao Xiaoa, Tingting Jia, Xinyuan Wanga, Yixuan Xua, Jianbo Xiaob,c,d,*, Hui Caoc,d, Zhiyong Chene, Huifan Liuf, Yuanqing Gaoa,*, Hongxun Taob,g,*
a Key Laboratory of Cardiovascular and Cerebrovascular Medicine, School of Pharmacy, Nanjing Medical University, Nanjing 211166, China
b School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
c Department of Analytical Chemistry and Food Science, Faculty of Science, University of Vigo, Vigo 36310, Spain
d CISPAC, Fontan Building, City of Culture, Santiago de Compostela 15707, Spain
e Shaanxi Academy of Traditional Chinese Medicine, Xi'an 710003, China
f Guangdong Key Laboratory of Science and Technology of Lingnan Specialty Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510550, China
g State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu 610075, China
*Corresponding author.
Abstract
Rhodiola crenulate is the edible medicinal herbal medicine widely used for altitude sickness in China. Interestingly, our previous work has found that R. crenulate extract (RCE) could significantly improve the pathology associated with dextran sulfate sodium-induced colitis. Thus, the current research aims to reveal the pharmacodynamic material basis of RCE, as well as its mechanism against colitis. The chemical characterization of RCE was performed by UHPLC-HR-MS, through which a total of 88 constituents were identified. Meanwhile, our results also found 29 constituents absorbed into blood and 8 metabolized absorbable compounds. The decreased flavonoids prototype and the elevated sulfated products of phenols were observed under pathophysiological conditions of colitis. The metabolomics study revealed that colitis caused the alternation of fatty acid metabolism, steroid hormone biosynthesis and bile acid metabolism. Correspondingly, RCE could prevent colitis by improving fatty acid metabolism and secondary bile acid metabolism.
PENG Y, XIAO X A, JI T T, et al. Reveal the pharmacodynamic substances and mechanism of an edible medicinal plant Rhodiola crenulate in DSS-induced colitis through plasma pharmacochemistry and metabolomics[J]. Food Science and Human Wellness, 2024, 13(4): 2116-2131. DOI:10.26599/FSHW.2022.9250176.
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