吴瑛（Jane Y. Wu）是一位开创性的神经科学家，她在西北大学的研究极大地推进了我们对神经发育、神经退行性疾病和癌症等领域的分子机制的理解。她的研究集中在几个关键领域，包括神经引导、RNA生物学以及线粒体功能的调控，这些都对该领域产生了深远的影响。
她的主要贡献之一是阐明了神经引导机制。吴博士研究了神经元如何通过响应细胞外信号迁移并形成复杂的网络，这对大脑的发育和功能至关重要。她的研究表明，这些引导分子不仅在神经系统中起着关键作用，而且在各种疾病（包括癌症转移）中也具有重要作用。
吴博士还在RNA结合蛋白（如TDP-43）的研究方面取得了重大进展，这些蛋白与ALS等神经退行性疾病有关。她揭示了这些蛋白如何调节microRNA，进而影响与癌症和神经退行性疾病相关的基因表达。她的研究显示，TDP-43在不同的背景下既可以促进也可以抑制癌症的进展，突显了其功能的复杂性。
此外，吴博士对线粒体动力学的研究揭示了线粒体功能与神经退行性疾病之间的复杂关系。她展示了线粒体蛋白质导入和质量控制机制如何与病理蛋白如TDP-43的降解相关联，为这些毁灭性疾病提供了潜在的治疗靶点。
通过对先进成像技术的创新运用以及对分子生物学的深刻理解，吴瑛的研究继续影响着神经科学领域，为大脑功能和疾病的基本过程提供了重要的见解。她的科学遗产展现了严谨的学术态度、创造力以及对揭开大脑复杂性的不懈追求。
饶议科学
华人生物学家和国际分子生物学家悼念吴瑛教授
吴瑛在美国西北大学发表的47篇论文：
1.Neuronal guidance genes in health and diseases.
Yuasa-Kawada J, Kinoshita-Kawada M, Tsuboi Y, Wu JY.
Protein Cell. 2023 Apr 21;14(4):238-261. doi: 10.1093/procel/pwac030.
PMID: 36942388 Free PMC article. Review.
2.Multi-molecular hyperspectral PRM-SRS microscopy.
Zhang W, Li Y, Fung AA, Li Z, Jang H, Zha H, Chen X, Gao F, Wu JY, Sheng H, Yao J, Skowronska-Krawczyk D, Jain S, Shi L.
Nat Commun. 2024 Feb 21;15(1):1599. doi: 10.1038/s41467-024-45576-6.
PMID: 38383552 Free PMC article.
3.Tongue as a first-line immune organ?
Wu JY.
Protein Cell. 2021 Mar;12(3):162-164. doi: 10.1007/s13238-020-00802-x. Epub 2021 Jan 9.
PMID: 33420957 Free PMC article. Review. No abstract available.
4.Super-resolution SRS microscopy with A-PoD.
Jang H, Li Y, Fung AA, Bagheri P, Hoang K, Skowronska-Krawczyk D, Chen X, Wu JY, Bintu B, Shi L.
Nat Methods. 2023 Mar;20(3):448-458. doi: 10.1038/s41592-023-01779-1. Epub 2023 Feb 16.
PMID: 36797410 Free PMC article.
5.Integration of FUNDC1-associated mitochondrial protein import and mitochondrial quality control contributes to TDP-43 degradation.
Ma J, Liu L, Song L, Liu J, Yang L, Chen Q, Wu JY, Zhu L.
Cell Death Dis. 2023 Nov 11;14(11):735. doi: 10.1038/s41419-023-06261-6.
PMID: 37951930 Free PMC article.
6.Detection of orthologous exons and isoforms using EGIO.
Ma J, Wu JY, Zhu L.
Bioinformatics. 2022 Sep 30;38(19):4474-4480. doi: 10.1093/bioinformatics/btac548.
PMID: 35946527 Free PMC article.
7.Autism, an area that needs public attention and investment.
Wu JY.
Sci China Life Sci. 2015 Oct;58(10):931-2. doi: 10.1007/s11427-015-4940-2.
PMID: 26490975 No abstract available.
8.TDP-43 regulates cancer-associated microRNAs.
Chen X, Fan Z, McGee W, Chen M, Kong R, Wen P, Xiao T, Chen X, Liu J, Zhu L, Chen R, Wu JY.
Protein Cell. 2018 Oct;9(10):848-866. doi: 10.1007/s13238-017-0480-9. Epub 2017 Sep 26.
PMID: 28952053 Free PMC article.
9.SLIT2/ROBO1-signaling inhibits macropinocytosis by opposing cortical cytoskeletal remodeling.
Bhosle VK, Mukherjee T, Huang YW, Patel S, Pang BWF, Liu GY, Glogauer M, Wu JY, Philpott DJ, Grinstein S, Robinson LA.
Nat Commun. 2020 Aug 17;11(1):4112. doi: 10.1038/s41467-020-17651-1.
PMID: 32807784 Free PMC article.
10.FUS Interacts with HSP60 to Promote Mitochondrial Damage.
Deng J, Yang M, Chen Y, Chen X, Liu J, Sun S, Cheng H, Li Y, Bigio EH, Mesulam M, Xu Q, Du S, Fushimi K, Zhu L, Wu JY.
PLoS Genet. 2015 Sep 3;11(9):e1005357. doi: 10.1371/journal.pgen.1005357. eCollection 2015 Sep.
PMID: 26335776 Free PMC article.
11.A new method for quantifying mitochondrial axonal transport.
Chen M, Li Y, Yang M, Chen X, Chen Y, Yang F, Lu S, Yao S, Zhou T, Liu J, Zhu L, Du S, Wu JY.
Protein Cell. 2016 Nov;7(11):804-819. doi: 10.1007/s13238-016-0268-3. Epub 2016 May 25.
PMID: 27225265 Free PMC article.
12.MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.
Wang F, Chen X, Cheng H, Song L, Liu J, Caplan S, Zhu L, Wu JY.
EMBO Rep. 2021 Jul 5;22(7):e52006. doi: 10.15252/embr.202052006. Epub 2021 Jun 6.
PMID: 34096155 Free PMC article.
13.Direct Imaging of Lipid Metabolic Changes in Drosophila Ovary During Aging Using DO-SRS Microscopy.
Li Y, Bagheri P, Chang P, Zeng A, Hao J, Fung A, Wu JY, Shi L.
Front Aging. 2022 Feb 3;2:819903. doi: 10.3389/fragi.2021.819903. eCollection 2021.
PMID: 35822015 Free PMC article.
14.A Drosophila model for TDP-43 proteinopathy.
Li Y, Ray P, Rao EJ, Shi C, Guo W, Chen X, Woodruff EA 3rd, Fushimi K, Wu JY.
Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3169-74. doi: 10.1073/pnas.0913602107. Epub 2010 Jan 26.
PMID: 20133767 Free PMC article.
15.Bioorthogonal Stimulated Raman Scattering Imaging Uncovers Lipid Metabolic Dynamics in Drosophila Brain During Aging.
Li Y, Chang P, Sankaran S, Jang H, Nie Y, Zeng A, Hussain S, Wu JY, Chen X, Shi L.
GEN Biotechnol. 2023 Jun 1;2(3):247-261. doi: 10.1089/genbio.2023.0017. Epub 2023 Jun 19.
PMID: 37363411 Free PMC article.
16.TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.
Wang P, Deng J, Dong J, Liu J, Bigio EH, Mesulam M, Wang T, Sun L, Wang L, Lee AY, McGee WA, Chen X, Fushimi K, Zhu L, Wu JY.
PLoS Genet. 2019 May 17;15(5):e1007947. doi: 10.1371/journal.pgen.1007947. eCollection 2019 May.
PMID: 31100073 Free PMC article.
17.A crucial role for Arf6 in the response of commissural axons to Slit.
Kinoshita-Kawada M, Hasegawa H, Hongu T, Yanagi S, Kanaho Y, Masai I, Mishima T, Chen X, Tsuboi Y, Rao Y, Yuasa-Kawada J, Wu JY.
Development. 2019 Feb 4;146(3):dev172106. doi: 10.1242/dev.172106.
PMID: 30674481 Free PMC article.
18.MiR-130a regulates neurite outgrowth and dendritic spine density by targeting MeCP2.
Zhang Y, Chen M, Qiu Z, Hu K, McGee W, Chen X, Liu J, Zhu L, Wu JY.
Protein Cell. 2016 Jul;7(7):489-500. doi: 10.1007/s13238-016-0272-7. Epub 2016 Jun 1.
PMID: 27245166 Free PMC article.
19.Traumatic injury induces stress granule formation and enhances motor dysfunctions in ALS/FTD models.
Anderson EN, Gochenaur L, Singh A, Grant R, Patel K, Watkins S, Wu JY, Pandey UB.
Hum Mol Genet. 2018 Apr 15;27(8):1366-1381. doi: 10.1093/hmg/ddy047.
PMID: 29432563 Free PMC article.
20.PINK1 and Parkin are genetic modifiers for FUS-induced neurodegeneration.
Chen Y, Deng J, Wang P, Yang M, Chen X, Zhu L, Liu J, Lu B, Shen Y, Fushimi K, Xu Q, Wu JY.
Hum Mol Genet. 2016 Dec 1;25(23):5059-5068. doi: 10.1093/hmg/ddw310.
PMID: 27794540 Free PMC article.
21.Noncanonical Myo9b-RhoGAP Accelerates RhoA GTP Hydrolysis by a Dual-Arginine-Finger Mechanism.
Yi F, Kong R, Ren J, Zhu L, Lou J, Wu JY, Feng W.
J Mol Biol. 2016 Jul 31;428(15):3043-57. doi: 10.1016/j.jmb.2016.06.014. Epub 2016 Jun 27.
PMID: 27363609 Free PMC article.
22.Reduced USP33 expression in gastric cancer decreases inhibitory effects of Slit2-Robo1 signalling on cell migration and EMT.
Xia Y, Wang L, Xu Z, Kong R, Wang F, Yin K, Xu J, Li B, He Z, Wang L, Xu H, Zhang D, Yang L, Wu JY, Xu Z.
Cell Prolif. 2019 May;52(3):e12606. doi: 10.1111/cpr.12606. Epub 2019 Mar 21.
PMID: 30896071 Free PMC article.
23.USP33 mediates Slit-Robo signaling in inhibiting colorectal cancer cell migration.
Huang Z, Wen P, Kong R, Cheng H, Zhang B, Quan C, Bian Z, Chen M, Zhang Z, Chen X, Du X, Liu J, Zhu L, Fushimi K, Hua D, Wu JY.
Int J Cancer. 2015 Apr 15;136(8):1792-802. doi: 10.1002/ijc.29226. Epub 2014 Sep 29.
PMID: 25242263 Free PMC article.
24.Axon guidance factor SLIT2 inhibits neural invasion and metastasis in pancreatic cancer.
Göhrig A, Detjen KM, Hilfenhaus G, Körner JL, Welzel M, Arsenic R, Schmuck R, Bahra M, Wu JY, Wiedenmann B, Fischer C.
Cancer Res. 2014 Mar 1;74(5):1529-40. doi: 10.1158/0008-5472.CAN-13-1012. Epub 2014 Jan 21.
PMID: 24448236
25.Detection of Dendritic Spines Using Wavelet-Based Conditional Symmetric Analysis and Regularized Morphological Shared-Weight Neural Networks.
Wang S, Chen M, Li Y, Zhang Y, Han L, Wu J, Du S.
Comput Math Methods Med. 2015;2015:454076. doi: 10.1155/2015/454076. Epub 2015 Nov 24.
PMID: 26692046 Free PMC article.
26.DSCAM functions as a netrin receptor in commissural axon pathfinding.
Liu G, Li W, Wang L, Kar A, Guan KL, Rao Y, Wu JY.
Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2951-6. doi: 10.1073/pnas.0811083106. Epub 2009 Feb 5.
PMID: 19196994 Free PMC article.
27.Morphological analysis of dendrites and spines by hybridization of ridge detection with twin support vector machine.
Wang S, Chen M, Li Y, Shao Y, Zhang Y, Du S, Wu J.
PeerJ. 2016 Jul 20;4:e2207. doi: 10.7717/peerj.2207. eCollection 2016.
PMID: 27547530 Free PMC article.
28.Neuron-to-Neuron Transfer of FUS in Drosophila Primary Neuronal Culture Is Enhanced by ALS-Associated Mutations.
Feuillette S, Delarue M, Riou G, Gaffuri AL, Wu J, Lenkei Z, Boyer O, Frébourg T, Campion D, Lecourtois M.
J Mol Neurosci. 2017 May;62(1):114-122. doi: 10.1007/s12031-017-0908-y. Epub 2017 Apr 20.
PMID: 28429234
29.An ALS-mutant TDP-43 neurotoxic peptide adopts an anti-parallel β-structure and induces TDP-43 redistribution.
Zhu L, Xu M, Yang M, Yang Y, Li Y, Deng J, Ruan L, Liu J, Du S, Liu X, Feng W, Fushimi K, Bigio EH, Mesulam M, Wang C, Wu JY.
Hum Mol Genet. 2014 Dec 20;23(25):6863-77. doi: 10.1093/hmg/ddu409. Epub 2014 Aug 11.
PMID: 25113748 Free PMC article.
30.FUS interacts with ATP synthase beta subunit and induces mitochondrial unfolded protein response in cellular and animal models.
Deng J, Wang P, Chen X, Cheng H, Liu J, Fushimi K, Zhu L, Wu JY.
Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):E9678-E9686. doi: 10.1073/pnas.1806655115. Epub 2018 Sep 24.
PMID: 30249657 Free PMC article.
31.Increased dysbindin-1B isoform expression in schizophrenia and its propensity in aggresome formation.
Xu Y, Sun Y, Ye H, Zhu L, Liu J, Wu X, Wang L, He T, Shen Y, Wu JY, Xu Q.
Cell Discov. 2015 Nov 10;1:15032. doi: 10.1038/celldisc.2015.32. eCollection 2015.
PMID: 27462430 Free PMC article.
32.Midline crossing and Slit responsiveness of commissural axons require USP33.
Yuasa-Kawada J, Kinoshita-Kawada M, Wu G, Rao Y, Wu JY.
Nat Neurosci. 2009 Sep;12(9):1087-9. doi: 10.1038/nn.2382. Epub 2009 Aug 16.
PMID: 19684588 Free PMC article.
33.CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development.
Tu T, Zhang C, Yan H, Luo Y, Kong R, Wen P, Ye Z, Chen J, Feng J, Liu F, Wu JY, Yan X.
Cell Res. 2015 Mar;25(3):275-87. doi: 10.1038/cr.2015.15. Epub 2015 Feb 6.
PMID: 25656845 Free PMC article.
34.Progranulin promotes neurite outgrowth and neuronal differentiation by regulating GSK-3β.
Gao X, Joselin AP, Wang L, Kar A, Ray P, Bateman A, Goate AM, Wu JY.
Protein Cell. 2010 Jun;1(6):552-62. doi: 10.1007/s13238-010-0067-1. Epub 2010 Jul 7.
PMID: 21204008 Free PMC article.
35.Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation.
Kinoshita-Kawada M, Hasegawa H, Hongu T, Yanagi S, Kanaho Y, Masai I, Mishima T, Chen X, Tsuboi Y, Rao Y, Yuasa-Kawada J, Wu JY.
Bio Protoc. 2019 Sep 20;9(18):e3373. doi: 10.21769/BioProtoc.3373. eCollection 2019 Sep 20.
PMID: 33654869 Free PMC article.
36.Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration.
Yuasa-Kawada J, Kinoshita-Kawada M, Rao Y, Wu JY.
Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14530-5. doi: 10.1073/pnas.0801262106. Epub 2009 Aug 12.
PMID: 19706539 Free PMC article.
37.Netrin signal transduction and the guanine nucleotide exchange factor DOCK180 in attractive signaling.
Li X, Gao X, Liu G, Xiong W, Wu J, Rao Y.
Nat Neurosci. 2008 Jan;11(1):28-35. doi: 10.1038/nn2022. Epub 2007 Dec 9.
PMID: 18066058 Free PMC article.
38.Polypyrimidine tract-binding protein (PTB) differentially affects malignancy in a cell line-dependent manner.
Wang C, Norton JT, Ghosh S, Kim J, Fushimi K, Wu JY, Stack MS, Huang S.
J Biol Chem. 2008 Jul 18;283(29):20277-87. doi: 10.1074/jbc.M803682200. Epub 2008 May 22.
PMID: 18499661 Free PMC article.
39.PSF suppresses tau exon 10 inclusion by interacting with a stem-loop structure downstream of exon 10.
Ray P, Kar A, Fushimi K, Havlioglu N, Chen X, Wu JY.
J Mol Neurosci. 2011 Nov;45(3):453-66. doi: 10.1007/s12031-011-9634-z. Epub 2011 Sep 1.
PMID: 21881826 Free PMC article.
40.RBM4 interacts with an intronic element and stimulates tau exon 10 inclusion.
Kar A, Havlioglu N, Tarn WY, Wu JY.
J Biol Chem. 2006 Aug 25;281(34):24479-88. doi: 10.1074/jbc.M603971200. Epub 2006 Jun 15.
PMID: 16777844 Free PMC article.
41.The splicing factor Prp31 is essential for photoreceptor development in Drosophila.
Ray P, Luo X, Rao EJ, Basha A, Woodruff EA 3rd, Wu JY.
Protein Cell. 2010 Mar;1(3):267-74. doi: 10.1007/s13238-010-0035-9. Epub 2010 Apr 17.
PMID: 21203973 Free PMC article.
42.Expression of human FUS/TLS in yeast leads to protein aggregation and cytotoxicity, recapitulating key features of FUS proteinopathy.
Fushimi K, Long C, Jayaram N, Chen X, Li L, Wu JY.
Protein Cell. 2011 Feb;2(2):141-9. doi: 10.1007/s13238-011-1014-5. Epub 2011 Feb 14.
PMID: 21327870 Free PMC article.
43.Preparation of intact mitochondria using free-flow isoelectric focusing with post-pH gradient sample injection for morphological, functional and proteomics studies.
He YC, Kong FZ, Fan LY, Wu JY, Liu XP, Li J, Sun Y, Zhang Q, Yang Y, Wu XJ, Xiao H, Cao CX.
Anal Chim Acta. 2017 Aug 22;982:200-208. doi: 10.1016/j.aca.2017.06.007. Epub 2017 Jun 17.
PMID: 28734361
44.Up-regulation of the proapoptotic caspase 2 splicing isoform by a candidate tumor suppressor, RBM5.
Fushimi K, Ray P, Kar A, Wang L, Sutherland LC, Wu JY.
Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15708-13. doi: 10.1073/pnas.0805569105. Epub 2008 Oct 7.
PMID: 18840686 Free PMC article.
45.SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy.
Wu JY, Kar A, Kuo D, Yu B, Havlioglu N.
Mol Cell Biol. 2006 Sep;26(18):6739-47. doi: 10.1128/MCB.00739-06.
PMID: 16943417 Free PMC article.
46.RNA helicase p68 (DDX5) regulates tau exon 10 splicing by modulating a stem-loop structure at the 5' splice site.
Kar A, Fushimi K, Zhou X, Ray P, Shi C, Chen X, Liu Z, Chen S, Wu JY.
Mol Cell Biol. 2011 May;31(9):1812-21. doi: 10.1128/MCB.01149-10. Epub 2011 Feb 22.
PMID: 21343338 Free PMC article.
47.p130CAS is required for netrin signaling and commissural axon guidance.
Liu G, Li W, Gao X, Li X, Jürgensen C, Park HT, Shin NY, Yu J, He ML, Hanks SK, Wu JY, Guan KL, Rao Y.
J Neurosci. 2007 Jan 24;27(4):957-68. doi: 10.1523/JNEUROSCI.4616-06.2007.
PMID: 17251438 Free PMC article.
