2024年9月20日，中国农业科学院油料作物研究所油料品质化学与加工利用创新团队在食品领域TOP期刊《Journal of Food Composition and Analysis》（IF:4）上发表了题为“Comprehensive lipid profile analysis of three fish roe by untargeted lipidomics using ultraperformance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry”的研究性论文。王杰和俞喜娜为本文的共同第一作者，魏芳研究员为本文通讯作者。
创新点
研究背景
研究内容
Fig. 1. Lipidomics analysis in roe samples from different fish species. (A) Total ion chromatogram (TIC) of the lipid profile acquired using UPLC Q-TOF-MS/MS (ESI+ and ESI− ). (B) Lipid subclasses identified in three fish roe. Number of lipids in each subclass. (C) Distribution of lipid subclasses identified in three fish roe. (D) Contents of total FA, SL, GL, and PL species three fish roe. (E) Contents of each lipid category in three fish roe.
Fig. 2. GL analysis in roe samples from different fish species. (A) Distribution of total carbon and double bond number of DAG in three fish roe (B) Percentage of various n-3 PUFA-TAG content in three fish roe. (C) Contents of individual EPA-DAG, DHA-DAG, DPA-DAG, ALA-DAG, EPA-TAG, and DHA-TAG in three fish roe.
Fig. 3. PL analysis in roe samples from different fish species. (A) Percentage of EPA-PL, DHA-PL, ALA-PL, and DPA-PL of the whole total PL content in three fish roe. (B) Content of EPA-PL, DHA-PL, ALA-PL, DPA-PL, and total PL in three fish roe. (C) Content of individual DPA-PC, DPA-PE, and ALA-PE in three fish roe. (D) Content of individual DHA-LPC, DHA-PC, DHA-PE, DHA-PG, DHA-PI, and DHA-PS in three fish roe. (E) Content of individual EPA-LPC, EPA-LPE, EPA-PC, EPA-PE, EPA-PG, EPA-PI, and EPA-PS in three fish roe. (F) Distribution of EPA-PC and DHA-PC molecular species in three fish roe. (G) Distribution of EPA-PE, DHA-PE, and DPA-PE molecular species in three fish roe.
Fig. 4. Multivariate statistical analysis in roe samples from different fish species. (A) 3D-PCA score plot of all lipid species in three fish roe. (B) PCA score plot of all lipid species in three fish roe. (C) VIP scores of individual lipids in PLS-DA. (D) Heat map analysis of 31 differential lipids in three groups. (E) Volcano plot of lipids between the mackerel roe group and the yellow croaker roe group. (F) Volcano plot of lipids between the capelin roe group and the yellow croaker roe group. (G) Volcano plot of lipids between the mackerel roe group and the capelin roe group.
结论
本研究利用无靶向脂质组学技术，对三种鱼籽中的脂质分子物种进行了深入的分析。共鉴定出449种脂质物种，涵盖了13个亚类。研究结果揭示了不同鱼籽在SLs、PLs和GLs方面的显著差异，为海洋鱼籽的营养价值提供了新的见解，并为海洋鱼副产品的加工提供了理论基础。
END
供稿：王杰
责任编辑：魏芳
论文链接：https://doi.org/10.1016/j.jfca.2024.106782
