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前言
| 植物嫁接的相容性是指两种植物形成连通维管束的连接能力。番茄和辣椒是相互不相容的嫁接伙伴；然而，这两种物种之间嫁接排斥的潜在原因仍然未知。
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Thomas et al 通过生物物理稳定性减弱、生长减少以及使用 trypan 蓝和 TUNEL 检测持续的细胞死亡，诊断了番茄和不同辣椒品种之间的嫁接不相容性。
PepMato 嫁接不相容，这意味着连接处的维管束组织无法愈合，导致茎部不稳定生长缓慢。
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嫁接者在不相容的树木连接处观察到了坏死组织。作者通过番茄-辣椒嫁接实验表明，这种情况也同样存在。甚至在嫁接后长达 3 周的时间里，仍然可以在连接处观察到细胞死亡区域！
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我们还发现所有嫁接的植株都具有程序性细胞死亡的升高水平，但是通过活性染色和 PCD 标记观察到的细胞死亡区域之间存在相当大的重叠。
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An extra line briefly explaining the TUNEL method would be
appreciated, just indicating that it measures fragmented DNA, which is a
proxy for cell viability. This is a great figure and a
well executed experiment. We wondered, however, if given that the
conclusions are negative this figure could potentially be supplementary.
Question
to the authors, outside of the scope of the study: Instead of growing
the calli and applying the exudates ectopically, would it be possible to
apply plant exudates directly on graft junctions somehow? For example,
by wrapping the graft with the exudate, like people do with hormones.
Applying the DAMP exudates from incompatible hetero-grafts to
self-grafts which are compatible would be interesting. This might be
technically more challenging but it might be more biologically relevant
than the callus-based assay. |
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不相容嫁接体中细胞死亡的原因是什么呢？作者利用 RNA-seq 技术进行了深入研究！结果发现，与相容的自体嫁接相比，不相容嫁接表现出持续的转录反应。
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- This is a super interesting experiment. We noted that
although there's a lot of downregulated genes identified, the mansucript
mostly discusses the upregulated ones. It's a shame because several NLR
or immune-related genes might be in the downregulated gene group. For
example,there may be downregulation of immune associated genes to for a
successful graft to happen,
- Related to this,
downregulated genes are not mentioned or analysed in any of the RNAseq
analyses performed in the manuscript. Why is this? There could be some
interesting information hiding in that set of downregulated genes.
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RNA-seq 分析揭示了不相容反应的真相！作者发现这种反应显著富集了防御过程相关的基因。此外，作者还在不相容嫁接体中鉴定出 1000 多个 NLR 基因和超敏反应/程序性细胞死亡关键成分的上调！
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- A main point discussed when reviewing the paper was that
given so many NLRs were identified, we would have liked a more thorough
description of the NLRs involved. What are the NLRs found? In solanaceae
one would focus on CC-NLRs and on the NRC immune receptor network which
can make up to 50% of the NLRome of solanaceous species.
- Although
SAG101, EDS1 are considered marker genes for immunity, they are not the
actual executors of PCD for all pathways. Highlighting helper NLR
families would be very interesting.
- What defines the order of the samples in the heatmap. Is it phylogenetic? Is it by gene ID?
- Organizing
the heatmap data by NLR family/category would potentially reveal
interesting patterns. For example, organizing the NLRs phylogenetically
according to their NB-ARC domain phylogeny/according to their N-terminal
executor domain (CC-NLR, TIR-NLR, etc…) would be super useful. Perhaps
all the upregulated NLRs belong to a specific phylogenetic clade, for
example.
- Classifying NLRs into Sensor and Helper NLRs
would be interesting too. Perhaps the cell death is triggered by an
instance of sensor-helper incompatibility?
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不相容的番茄嫁接体中调控上调的一组基因非常酷炫：
BREAST CANCER SUSCEPTIBILITY GENE 1 (BRCA1) and BRCA1 ASSOCIATED RING DOMAIN PROTEIN 1。没错，您没看错！这些基因参与 DNA 修复机制，在植物中也并不常见！
番茄和辣椒似乎在基因上不相容！这种不相容会导致 NLR 上调、DNA 损伤和细胞死亡。这让人联想到了什么？
自身免疫反应! AUTOIMMUNITY!
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基于这项研究，我们认为番茄和辣椒在基因上不相容，并触发了一种超强免疫反应！
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原创深度解析：移动mRNA，双链RNA受体，蛋白翻译参与的植物免疫，TOR信号与再生，CaMV，TOR与光信号，TOR与RNA可变剪切，
客户文章精选：TOR-4EBP，荔枝脱落，CUCUME数据库，伴侣蛋白辅助RNP运输，二价染色质与环境响应。
学术争鸣：COOLAIR，PRC2，CDKs，细胞内运输，
原创锐评：Science子刊错误的数据分析，Nature糟糕的审稿，Elsevier的贪婪，经常被忽略的植物学研究，顶刊忽视预印，拉大牛入伙，科研评价体系，一稿多投，让人失望的Plant Journal，Current Biology发文良莠不齐。
写作指导：标题，cover letter，摘要，图和图注，讨论，回复审稿人，润色。
