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薯蓣皂苷抑制肿瘤生长
图1 薯蓣皂苷（24 mg/kg）抑制异种移植模型的肿瘤生长
显著差异表达miRNA的靶基因的功能注释和富集分析
薯蓣皂苷诱导细胞凋亡和自噬
图4 薯蓣皂苷调节Ishikawa细胞凋亡及自噬蛋白的表达
薯蓣皂苷对PI3K/AKT和p53信号通路的影响
图5 免疫印迹分析PI3K/AKT/mTOR信号通路蛋白
薯蓣皂苷诱导细胞自噬和细胞凋亡之间的关系
图7 薯蓣皂苷诱导的Ishikawa细胞自噬与凋亡的关系
miRNA和mRNA的验证和调控
Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo
Xiaoli Lia,b,c, Runhui Maa,b, Zhijing Nia, Wei Wanga, Kiran Thakura,b, Jianguo Zhanga,b,*, Zhaojun Weia,b,*
a School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China
b School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China
c School of Public Health, Anhui University of Science and Technology, Huainan 232001, China
*Corresponding author.
Abstract
With an aim to comprehend the precise regulatory mechanism of dioscin against endometrial carcinoma (EC), we firstly extracted the components from Polygonatum sibiricum followed by identification and structural characterization. The anti-EC activity of dioscin was initially determined based on the inhibition of Ishikawa cell proliferation and tumor growth. The high-throughput sequencing data indicated that dioscin not only promoted apoptosis, including decrease of poly ADP-ribose polymerase (PARP) and B-cell lymphoma-2 (Bcl-2) and increase of c-PARP and Bcl-2-associcated agonist of cell death (Bad), but also induced autophagy, including increase of autophagic lysosomes and LC3Ⅱ/LC3Ⅰ ratio. Mechanistic exploration suggested that dioscin induced autophagy and apoptosis through inhibition of PI3K/AKT/mTOR signaling pathway. Besides, the dioscin-regulated p53 pathway was mainly involved in autophagy induction. Furthermore, inhibition of Ishikawa cell autophagy was linked to dioscin-induced apoptosis. Our data suggest the immense potential of dioscin for the development of functional food for EC and related medical application.
Reference:
LI X L, MA R H, NI Z J, et al. Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo[J]. Food Science and Human Wellness, 2024, 13(5): 2601-2616. DOI:10.26599/FSHW.2022.9250209.
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