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肠上皮细胞作为肠道免疫系统的第一道防线，在维持免疫稳态、发挥免疫防御功能、平衡损伤修复等方面起着至关重要的作用。从这个意义上说，选择CMT93肠道细胞作为研究对象，研究外源性组胺对肠道免疫防御系统第一道防线的影响。首先用不同浓度的组胺处理细胞。结果发现，与对照组相比，用0～5 mmol/L组胺处理的细胞活力没有变化。因此，选择这些浓度水平的组胺进行进一步研究，以消除组胺的细胞毒性作用的影响。采用RT-qPCR检测CMT93分泌的胸腺基质淋巴细胞生成素（TSLP）、IL-25和IL-33等上皮免疫细胞因子的表达。如图2所示，高剂量组胺刺激后TSLP、IL-25和IL-33的表达均上调。IL-33在1.00 mmol/L组胺刺激下显著增强（图2B），而IL-25和TSLP在5.00 mmol/L组胺刺激下显著上调（图2A和C）。这可能是由于不同细胞因子对组胺浓度的敏感性不同。这些结果表明，外源性组胺可以影响结肠上皮细胞中免疫相关转录因子的表达。
组胺对免疫细胞亚群的影响
组胺对树突状细胞和T细胞细胞因子的影响
Immunological disturbance effect of exogenous histamine towards key immune cells
Yanan Liua, Huan Lia, Chong Wanga, Shanjun Chena, Renjie Lianb, Weiqiang Wangc, Linglin Fua, Yanbo Wanga,d,*
a Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China
b Jinghai Group Co., Ltd., Rongcheng 264307, China
c Jinhua Jinnian Ham Co., Ltd., Jinhua 321041, China
d School of Food and Health, Beijing Technology and Business University, Beijing 100048, China
*Corresponding author.
Abstract
Histamine in food has attracted widespread attention due to the potential toxicity and associated health risk. However, its influences on immunological components, especially the function of key immune cells, are still poorly known. In this work, we explored the effects of exogenous histamine on the function of key immune cells such as intestinal epithelial cells, dendritic cells, and T cells. The results showed that histamine could affect the expression of allergy-related genes in CMT93 cells at a high dose of histamine. Moreover, it’s found that histamine could cause an imbalance in the levels of relevant immune factors secreted by dendritic cells and T cells, especially those related to allergy. At the same time, the proportion of MHC class Ⅱ-positive dendritic cells and the proportion of T helper 2 (Th2) cells in CD4+ T cells increased after histamine stimulation. We concluded that the presence of a certain level of histamine in food may affect the expression of allergy-related cytokines, disrupt the balance of the immune homeostasis, and potentially lead to adverse immune reactions. This work demonstrated the importance of including the estimation of histamine’s immune safety in aquatic products rather than merely considering the potential risk of food poisoning.
LIU Y N, LI H, WANG C, et al. Immunological disturbance effect of exogenous histamine towards key immune cells[J]. Food Science and Human Wellness, 2024, 13(4): 1856-1863. DOI:10.26599/FSHW.2022.9250154.
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