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本研究通过OVA诱导小鼠模型，研究活的Probio-M8和热灭活的Probio-M8对食物过敏的影响（图1）。与NC组相比，OVA处理小鼠血清OVA- sIgE和组胺水平显著升高。M8治疗显著降低血清中OVA致敏诱导的OVA- sIgE和组胺生成。HE染色的空肠组织显示，NC组粘膜层和粘膜下层均正常。相反，OVA诱导的小鼠空肠组织表现为空肠黏膜破坏、隐窝丢失和绒毛高度缩短。然而，M8治疗维持了空肠组织的完整性。总之，这些发现表明，活的M8抑制了OVA诱导的食物过敏小鼠的疾病发展。
图1 M8对血清中OVA-sIgE和组胺水平及空肠组织形态的影响
图2 M8对细胞因子的影响
图3 M8对肠道菌群的影响
史佳鹭，女，东南大学公共卫生学院，营养与食品卫生学系在读博士研究生，主要从事食物过敏与肠道微生态、功能特性益生菌的开发与应用、短链脂肪酸对肠黏膜稳态免疫调节作用等方面的研究。作为学术骨干参与国家重点研发计划青年科学家项目、国家自然科学基金、江苏省自然科学基金等国家省部级项目6项，在国内外期刊公开发表学术论文20余篇，其中以第一作者发表SCI 论文6篇，核心3篇，获得授权发明专利1项。
Preventive effects of Bifidobacterium lactis Probio-M8 on ovalbumin-induced food allergy in mice
Jialu Shia, Yan Xua, Cheng Liua, Shizhi Wanga,*, Jin Wanga,*, Vijaya Raghavanb,*
a Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China
b Department of Bioresource Engineering, Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, QC H9X3V9, Canada
*Corresponding author.
Abstract
Food allergy is a significant public health concern globally. Certain probiotics have been found to enhance food allergy by regulating immune-microbe interactions in animal models and patients. However, the effects of Bifidobacterium lactis Probio-M8 on food allergy have not been thoroughly investigated. The present study examined the anti-allergic properties of Probio-M8, particularly in relation to immune response and gut microbiota composition. Results demonstrate that oral administration of Probio-M8 effectively mitigated the allergy symptoms triggered by ovalbumin (OVA) by ameliorating the morphological damage in the jejunum, reducing OVA-specific IgE and histamine levels in the serum, and suppressing Th2 cytokines (interleukin (IL) 4 and IL-13) while increasing Th1 cytokines (interferon (IFN) γ) and regulatory T (Treg) cytokines (IL-10 and transforming growth factor (TGF) β1) in the culture supernatants of splenic cells. Furthermore, Probio-M8 effectively altered the diversity and composition of gut microbiota, particularly the relative abundances of Akkermansia_muciniphila in OVA-induced mice. Compared to the OVA group, the Probio-M8 group showed a decrease in the relative abundance of Akkermansia_muciniphila. In conclusion, Probio-M8 demonstrates the potential to alleviate food allergy by regulating the Th1/Th2 response and modulating gut microbiota, thereby offering a novel therapeutic strategy for patients with food allergy.
SHI J L, XU Y, LIU C, et al. Preventive effects of Bifidobacterium lactis Probio-M8 on ovalbumin-induced food allergy in mice[J]. Food Science and Human Wellness, 2024, 13(4): 2346-2352. DOI:10.26599/FSHW.2022.9250250.
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