人、小鼠、大鼠常见细胞比例参考值
Table 2. Human cell frequency - peripheral blood
| Common Leukocytes | Lymphoid Cells | Myeloid Cells | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Cell Type | Percent | Cell Type | Percent | Cell Type | Percent | |||||
T cells | 10-25 | CD4+ | 10-20 | Neutrophils | 40-65 | |||||
B cells | 3-10 | CD8+ | 3-10 | Eosinophils | 0.1-2 | |||||
Granulocytes | 45-65 | Tregs | 0.2-1.4 | Basophils | 0.1-2 | |||||
Monocytes | 3-10 | γδ T cells | 1-5 | Dendritic cells (DC) | 0.5-1 | |||||
NK cells | 2-5 | Naïve B cells | 0.5-5 | Monocyte DCs | 0.2-0.4 | |||||
Dendritic cells (DC) | 0.5-1 | Memory B cells | 0.4-2 | Plasmacytoid DCs | 0.1-0.2 | |||||
Stem cells | 0.01-0.05 | NKT cells | 0.01-0.5 | Innate lymphoid cells | 0.01-0.1 | |||||
Table 3. Murine cell frequency
| Spleen | Thymus | Peripheral Blood | Lymph Node | ||||
|---|---|---|---|---|---|---|---|
| Cell Type | Percent | Cell Type | Percent | Cell Type | Percent | Cell Type | Percent |
T cells | 21-35 | CD4+ | 4-6 | T cells | 17-20 | T cells | 65-78 |
CD4+ | 13-20 | CD8+ | 1-2 | CD4+ | 8-12 | CD4+ | 35-64 |
CD8+ | 7-15 | CD4+/8+ | 85-95 | CD8+ | 7-10 | CD8+ | 19-30 |
γδ T cells | 0.5-1 |
|
| B cells | 35-58 | B cells | 9-15 |
B cells | 44-58 |
|
| Monocytes | 2-3 | Tregs | 2-3 |
Monocytes | 3.5-5 |
|
| Neutrophils | 4-6 | Dendritic cells | 0.5-1 |
Granulocytes | 1-2 |
|
| Eosinophils | 1-2 |
|
|
NK cells | 1-5 |
|
| NK cells | 4-7 |
|
|
iNKT | 1-2 |
|
| iNKT cells | 0.2-0.5 |
|
|
Myeloid DC | 0.5-2 |
|
|
|
|
|
|
Lymphoid DC | 0.5-1 |
|
|
|
|
|
|
Plasmacytoid DC | 0.1-0.3 |
|
|
|
|
|
|
Table 4. Rat cell frequency
| Spleen | Thymus | Peripheral Blood | |||
|---|---|---|---|---|---|
Cell Type | Percent | Cell Type | Percent | Cell Type | Percent |
T cells | 31.5-33.5 | CD4+ | 5.7-9.4 | T cells | 51.5-66.5 |
CD4+ | 21.5-26.5 | CD8+ | 2.3-6.9 | CD4+ | 32-40 |
CD8+ | 14-18 | CD4+/8+ | 83-87.6 | CD8+ | 18-26 |
B cells | 51-59 | CD4-/8- | 1.4-2.2 | B cells | 22.7-40.7 |
|
|
|
| Monocytes | 1-6 |
|
|
|
| Neutrophils | 14-20 |
|
|
|
| Eosinophils | 1-4 |
|
|
|
| Basophils | Rare |
|
|
|
| γδ T cells | 1-2 |
|
|
|
| NK cells | 6-10 |
https://www.bio-rad-antibodies.com/flow-cytometry-cell-frequency.htmlhttps://www.jingege.wang/2022/01/17/%e4%ba%ba%e3%80%81%e5%b0%8f%e9%bc%a0%e3%80%81%e5%a4%a7%e9%bc%a0%e5%b8%b8%e8%a7%81%e7%bb%86%e8%83%9e%e6%af%94%e4%be%8b%e5%8f%82%e8%80%83%e5%80%bc/常见流式实验、免疫实验https://assets.thermofisher.cn/TFS-Assets/BID/Handbooks/flow-cytometry-protocols-handbook-zh.pdf
