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Abstract
背景回顾：The phytohormone auxin plays a pivotal role in governing plant growth and development.
提出问题：Although the TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX (TIR1/AFB) receptors function in both the nucleus and cytoplasm, the mechanism governing the distribution of TIR1/AFBs between these cellular compartments remains unknown.
主要发现：In this study, we demonstrate that auxin-mediated oxidation of TIR1/AFB2 is essential for their targeting to the nucleus.
结果1-FER-LLG1-ROP2-RBOHC-ROS/NO-TIR1/AFB2入核：We showed that small active molecules, reactive oxygen species (ROS) and nitric oxide (NO), are indispensable for the nucleo-cytoplasmic distribution of TIR1/AFB2 in trichoblasts and root hairs. Further studies revealed that this process is regulated by the FERONIA receptor kinase-NADPH oxidase signaling pathway.
结果2-ROS/NO-TIR1/AFB2氧化修饰-入核-生长素响应： Interestingly, ROS and NO initiate oxidative modifications in TIR1C140/516 and AFB2C135/511, facilitating their subsequent nuclear import. The oxidized forms of TIR1C140/516 and AFB2C135/511play a crucial role in enhancing the function of TIR1 and AFB2 in transcriptional auxin responses.
结论：Collectively, our study reveals a novel mechanism by which auxin stimulates the transport of TIR1/AFB2 from the cytoplasm to the nucleus, orchestrated by the FERONIA-ROS signaling pathway.
摘 要
植物激素生长素在植物生长和发育过程中发挥重要作用。 尽管目前已知TIR1/AFB受体在细胞核和细胞质中发挥功能，但是TIR1/AFB在胞内区室间的分布机制还不清楚。 本文中，作者发现生长素介导的TIR1/AFB2蛋白氧化修饰对于其核定位是必要的。 作者发现活性氧ROS和一氧化氮NO等小的活性分子对于根毛原细胞（trichoblasts）和根毛中TIR1/AFB2的细胞核-质分布是不可或缺的。 进一步的研究显示该过程受到FERONIA受体激酶-NADPH氧化酶信号转导途径的调控。 有趣的是，ROS和NO会起始TIR1（Cys140/156）和AFB2（Cys135/511）蛋白的氧化修饰，从而进一步促进TIR1和AFB2的入核。 TIR1和AFB2蛋白的氧化修饰能够极大增强其介导的生长素转录响应。 综上，本文的研究揭示了一个由生长素促进TIR1/AFB2由细胞质入细胞核，且受到FERONIA-ROS信号转导途径调控的新机制。
华东师范大学李超研究员为本文通讯作者，逯佰艳博士为本文第一作者。 该研究得到了国家自然科学基金（32230009、31770307、31972863）和上海科委科技创新行动计划（20ZR1416500）的联合资助。
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