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Abstract
背景回顾：Plants have an astonishing ability to regenerate new organs after wounding.
主要发现：Here, we report that the wound-inducible transcription factor ENHANCER OF SHOOT REGENERATION1 (ESR1) has a dual mode of action in activating ANTHRANILATE SYNTHASE ALPHA SUBUNIT1 (ASA1) expression to ensure auxin-dependent de novo root organogenesis locally at wound sites of Arabidopsis (Arabidopsis thaliana) leaf explants.
结果1-模式一：In the first mode, ESR1 interacts with HISTONE DEACETYLASE6 (HDA6), and the ESR1–HDA6 complex directly binds to the JASMONATE-ZIM DOMAIN5 (JAZ5) locus, inhibiting JAZ5 expression through histone H3 deacetylation. As JAZ5 interferes with the action of ETHYLENE RESPONSE FACTOR109 (ERF109), the transcriptional repression of JAZ5 at the wound site allows ERF109 to activate ASA1 expression.
结果2-模式二：In the second mode, the ESR1 transcriptional activator directly binds to the ASA1 promoter to enhance its expression.
结论：Overall, our findings indicate that the dual biochemical function of ESR1, which specifically occurs near wound sites of leaf explants, maximizes local auxin biosynthesis and de novo root organogenesis in Arabidopsis.
摘 要
植物在创伤后能够再生出新的器官。本文中，作者发现受创伤诱导的转录因子ESR1对于激活ASA1的表达具有双重作用模式，从而确保拟南芥叶片外植体上创伤部位的生长素依赖性从头根器官再生。在第一种作用模式下，ESR1能够与组蛋白去乙酰酶互作，进而ESR1–HDA6复合体直接结合到JA信号途径抑制因子JAZ5基因位点上，并通过组蛋白H3去乙酰化抑制JAZ5的表达。由于JAZ5会干扰乙烯响应因子ERF109的作用，因此创伤位点处的JAZ5基因被转录抑制后会释放ERF109以激活ASA1的表达。在第二种模式下，ESR1转录激活子直接结合到ASA1基因的启动子上，增强ASA1基因的表达。综上，本文的研究揭示了ESR1的双重生化功能，其特异性地发生在叶片的创伤部位，从而最大化局部的生长素合成，促进从头根器官再生。
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