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Abstract
背景回顾：Plants frequently encounter wounding and have evolved an extraordinary regenerative capacity to heal the wounds.
提出问题：However, the wound signal that triggers regenerative responses has not been identified.
主要发现：Here, through characterization of a tomato mutant defective in both wound-induced defense and regeneration, we demonstrate that in tomato, a plant elicitor peptide (Pep), REGENERATION FACTOR1 (REF1), acts as a systemin-independent local wound signal that primarily regulates local defense responses and regenerative responses in response to wounding.
结果1-REF1-PORK1-再生：We further identified PEPR1/2 ORTHOLOG RECEPTOR-LIKE KINASE1 (PORK1) as the receptor perceiving REF1 signal for plant regeneration.
结果2-REF1-PORK1-WIND1-再生：REF1-PORK1-mediated signaling promotes regeneration via activating WOUND-INDUCED DEDIFFERENTIATION 1 (WIND1), a master regulator of wound-induced cellular reprogramming in plants.
结论：Thus, REF1-PORK1 signaling represents a conserved phytocytokine pathway to initiate, amplify, and stabilize a signaling cascade that orchestrates wound-triggered organ regeneration.
应用：Application of REF1 provides a simple method to boost the regeneration and transformation efficiency of recalcitrant crops.
摘 要
植物经常遭受创伤，并演化出一种非凡的再生能力来愈合伤口。然而，诱发再生响应的创伤信号还未被鉴定。本文中，作者通过对一个创伤诱导防御和再生缺陷的番茄突变体的研究，发现了番茄中一种植物激发子肽REF1作为一个独立于系统素的局部创伤信号发挥作用，主要调控植物对创伤的局部防御响应和再生响应。作者还发现PORK1是植物再生中感知REF1信号的受体。REF1-PORK1介导的信号转导通过激活植物创伤诱导的细胞重编程主要调控子WIND1来促进再生。因此，REF1-PORK1信号转导代表了一个保守的植物细胞因子途径，能够启动、放大和稳定一个信号转导级联，精细调控创伤诱导的器官再生。通过外施REF1为提高顽拗性作物的再生转化效率提供了一种简单的方法。
中科院遗传与发育生物学研究所李传友研究员和山东农业大学邓磊教授为本文共同通讯作者，博士研究生杨文韬、翟华伟副教授、吴芳明副研究员和邓磊教授为本文的共同第一作者。该研究得到了国家自然科学基金（31991183、32161133018和U22A20459）、国家重点研发计划（2022YFF1003000、2021YFF1000103和2022YFD1400800）和泰山番茄创新研究院的联合资助。
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