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Abstract
背景回顾：Clonal propagation of plants by induction of adventitious roots (ARs) from stem cuttings is a requisite step in breeding programs. A major barrier exists for propagating valuable plants that naturally have low capacity to form ARs. Due to the central role of auxin in organogenesis, indole-3-butyric acid is often used as part of commercial rooting mixtures, yet many recalcitrant plants do not form ARs in response to this treatment.
主要发现：Here we describe the synthesis and screening of a focused library of synthetic auxin conjugates in Eucalyptus grandis cuttings and identify 4-chlorophenoxyacetic acid–L-tryptophan-OMe as a competent enhancer of adventitious rooting in a number of recalcitrant woody plants, including apple and argan.
作用机制：Comprehensive metabolic and functional analyses reveal that this activity is engendered by prolonged auxin signaling due to initial fast uptake and slow release and clearance of the free auxin 4-chlorophenoxyacetic acid.
结论：This work highlights the utility of a slow-release strategy for bioactive compounds for more effective plant growth regulation.
摘 要
利用插条诱导不定根，从而进行无性繁殖，这是育种项目中一个必要程序。某些珍贵植物存在不定根形成困难的情况，从而限制了其无性繁殖。生长素在器官发生中具有重要作用，吲哚-3-丁酸（IBA）通常被用于生根粉的组成成分之一，但仍旧有很多顽拗性植物对于生根粉并不敏感。本文中，作者以巨桉插条为研究材料，合成并筛选了一个人工合成的生长素偶联物，鉴定到了一个叫做4-氯苯氧乙酸-L-色氨酸-OMe的物质，其能够增强苹果和坚果树等多种顽拗性木本植物的不定根形成。综合代谢和功能分析，作者发现4-氯苯氧乙酸-L-色氨酸-OMe通过开始快速吸收并固定4-氯苯氧乙酸（4-CPA，一种具有生长素活性的植物生长调节剂），然后缓慢释放自由态4-CPA，并且4-CPA的清除同样缓慢，从而延长了生长素信号转导，最终促进了不定根的形成。本研究揭示了生物活性材料的缓释策略是一种调控植物生长的行之有效手段。
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