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Abstract
背景回顾：Chromatin configuration is critical for establishing tissue identity and changes substantially during tissue identity transitions. The crucial scientific and agricultural technology of in vitro tissue culture exploits callus formation from diverse tissue explants and tissue regeneration via de novo organogenesis.
主要发现：We investigated the dynamic changes in H3ac and H3K4me3 histone modifications during leaf-to-callus transition in Arabidopsis thaliana.
结果1-图谱绘制：We analyzed changes in the global distribution of H3ac and H3K4me3 during the leaf-to-callus transition, focusing on transcriptionally active regions in calli relative to leaf explants, defined by increased accumulation of both H3ac and H3K4me3.
结果2-多肽信号：Peptide signaling was particularly activated during callus formation; the peptide hormones RGF3, RGF8, PIP1 and PIPL3 were upregulated, promoting callus proliferation and conferring competence for de novo shoot organogenesis. The corresponding peptide receptors were also implicated in peptide-regulated callus proliferation and regeneration capacity. The effect of peptide hormones in plant regeneration is likely at least partly conserved in crop plants.
结论：Our results indicate that chromatin-dependent regulation of peptide hormone production not only stimulates callus proliferation but also establishes pluripotency, improving the overall efficiency of two-step regeneration in plant systems.
摘 要
染色质构象对于建立组织身份至关重要，并且在组织身份转变过程中发生显著变化。体外组织培养的关键技术就是利用不同的组织外植体诱导愈伤并通过从头器官发生实现组织再生。作者研究了拟南芥叶片到愈伤转变过程中的H3ac和H3K4me3组蛋白修饰的动态变化。作者分析了H3ac和H3K4me3在叶片到愈伤转变过程中的整体分布，主要关注了相比于叶片外植体而在愈伤中转录激活的区域，这些区域的特征是积累更多的H3ac和H3K4me3修饰。研究发现，在愈伤形成过程中多台信号转导被明显激活，其中多肽激素RGF3、RGF8、PIP1以及PIPL3显著上调，促进了愈伤的增殖以及赋予其能够从头芽再生的能力。相应的多肽受体同样参与了由多肽介导的愈伤组织和再生能力调控。多肽激素在植物再生中的作用在作物中至少部分保守。本文的研究结果揭示了染色质依赖性的多肽激素合成调控不仅促进了愈伤增殖，同时还建立了多能性，全面提升了植物中通过“两步法”进行再生的效率。
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