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Abstract
背景回顾：The potential for totipotency exists in all plant cells; however, the underlying mechanisms remain largely unknown. Earlier findings have revealed that the overexpression of LEAFY COTYLEDON 2 (LEC2) can directly trigger the formation of somatic embryos on the cotyledons of Arabidopsis. Furthermore, cotyledon cells that overexpress LEC2 accumulate significant lipid reserves typically found in seeds.
提出问题：The precise mechanisms and functions governing lipid accumulation in this process remain unexplored.
主要发现：In this study, we demonstrate that WRINKLED1 (WRI1), the key regulator of lipid biosynthesis, is essential for somatic embryo formation, suggesting that WRI1-mediated lipid biosynthesis plays a crucial role in the transition from vegetative to embryonic development.
结果1-WRI1-LEC2：Our findings indicate a direct interaction between WRI1 and LEC2, which enhances the enrichment of LEC2 at downstream target genes and stimulates their induction.
结果2-WRI-LEC1/2/FUS3-YUC4/OLE3：Besides, our data suggest that WRI1 forms a complex with LEC1, LEC2, and FUSCA3 (FUS3) to facilitate the accumulation of auxin and lipid for the somatic embryo induction, through strengthening the activation of YUCCA4 (YUC4) and OLEOSIN3 (OLE3) genes.
结论：Our results uncover a regulatory module controlled by WRI1, crucial for somatic embryogenesis. These findings provide valuable insights into our understanding of plant cell totipotency.
摘 要
所有植物细胞都具有全能性潜力，但其潜在调控机制还不清楚。早先的研究显示过表达LEC2能够诱导拟南芥子叶上直接形成体细胞胚。此外，子叶细胞过表达LEC2基因会积累很多常见于种子中的油脂储备。该过程中有关于油脂积累的精确调控机制和功能并不清楚。本文中，作者发现油脂生物合成关键蛋白WRI1对于体细胞胚的形成是必须的，表明WRI1介导的油脂合成在植物从营养生长向胚胎发育的转变过程中发挥重要作用。作者的发现揭示了WRI1和LEC2之间存在直接的相互作用，进而增强了LEC2在其下游靶基因上的富集，从而刺激下游靶基因的表达。除此之外，作者的研究还显示WRI1能够与LEC1、LEC2以及FUS3形成复合体，从而通过激活生长素合成基因YUC4和油体蛋白编码基因OLE3的表达，促进体胚诱导所需要的生长素和油脂的积累。本文的研究结果揭示了一个由WRI1控制的、作用于植物体胚发生的调控模块。本文的发现为我们深入理解植物细胞全能性提供了珍贵的视野。
山东农业大学苏英华教授为论文通讯作者，张文杰博士和唐丽苹副教授为本文共同第一作者。该研究得到了国家自然科学基金项目和山东省自然科学基金的资助。
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