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Abstract
背景回顾：Gibberellic acid (GA) plays a central role in many plant developmental processes and is crucial for crop improvement. DELLA proteins, the core suppressors in the GA signaling pathway, are degraded by GA via the 26S proteasomal pathway to release the GA response.
提出问题：However, little is known about the phosphorylation-mediated regulation of DELLA proteins.
主要发现：In this study, we combined GA response assays with protein–protein interaction analysis to infer the connection between Arabidopsis thaliana DELLAs and the C-TERMINAL DOMAIN PHOSPHATASE-LIKE 3 (CPL3), a phosphatase involved in the dephosphorylation of RNA polymerase II.
结果：We show that CPL3 directly interacts with DELLA proteins and promotes DELLA protein stability by inhibiting its degradation by the 26S proteasome. Consequently, CPL3 negatively modulates multiple GA-mediated processes of plant development, including hypocotyl elongation, flowering time, and anthocyanin accumulation.
结论：Taken together, our findings demonstrate that CPL3 serves as a novel regulator that could improve DELLA stability and thereby participate in GA signaling transduction.
摘 要
赤霉素（GA）在很多植物发育进程中发挥核心作用，对于作物改良至关重要。DELLA蛋白是GA信号转导途径的核心抑制子，而GA通过26S蛋白酶体途径降解DELLA蛋白从而释放GA响应。但是，有关磷酸化介导的DELLA蛋白调控还了解甚少。本文中，作者结合了GA响应试验及蛋白-蛋白互作分析，推断了拟南芥DELLA蛋白与CPL3的关联，而CPL3是一个参与RNA聚合酶II去磷酸化的磷酸酶。作者发现CPL3能够直接与DELLA蛋白发生互作，并且通过抑制26S蛋白酶体介导的DELLA蛋白降解从而促进DELLA蛋白的稳定性。因此，CPL3负调控多个由GA介导的植物发育过程，包括了下胚轴伸长、开花时间以及花色苷积累。综上，本文的研究揭示了CPL3作为一个新的调控子能够提升DELLA蛋白稳定性，从而参与植物中的GA信号转导。
根特大学Dirk Inzé教授和Marieke Dubois博士为本文共同通讯作者，李婷教授为本文第一作者。该研究得到了根特大学（BOF08/01M00408）、中国国家自然科学基金（32100263）、四川省自然科学基金（23NSFSC0765）和Flanders研究基金会博士后奖学金（12Q7923N）的联合资助。
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