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Abstract
背景回顾：Although polyploid plants have lower stomatal density than their diploid counterparts, the molecular mechanisms underlying this difference remain elusive.
主要发现：Here, we constructed a network based on the triploid poplar transcriptome data and triple-gene mutual interaction algorithm and found that PpnMYC2 was related to stomatal development-related genes PpnEPF2, PpnEPFL4, and PpnEPFL9.
结果1-MYC2过表达表型：The interactions between PpnMYC2 and PagJAZs were experimentally validated. PpnMYC2-overexpressing poplar and Arabidopsis thaliana had reduced stomatal density. Poplar overexpressing PpnMYC2 had higher water use efficiency and drought resistance.
结果2-MYC2作用机制：RNA-sequencing data of poplars overexpressing PpnMYC2 showed that PpnMYC2 promotes the expression of stomatal density inhibitors PagEPF2 and PagEPFL4 and inhibits the expression of the stomatal density-positive regulator PagEPFL9. Yeast one-hybrid system, electrophoretic mobility shift assay, ChIP-qPCR, and dual-luciferase assay were employed to substantiate that PpnMYC2 directly regulated PagEPF2, PagEPFL4, and PagEPFL9.
结果3-MYC2与三倍体杨树：PpnMYC2, PpnEPF2, and PpnEPFL4 were significantly upregulated, whereas PpnEPFL9 was downregulated during stomatal formation in triploid poplar.
结论：Our results are of great significance for revealing the regulation mechanism of plant stomatal occurrence and polyploid stomatal density, as well as reducing stomatal density and improving plant water use efficiency by overexpressing MYC2.
摘 要
尽管多倍体植物相比于其二倍体具有更低的气孔密度，但是其分子调控机制仍不清楚。本文中，作者基于三倍体杨树转录组和三基因互作算法构建了一个网络，发现PpnMYC2与气孔发育相关基因PpnEPF2、PpnEPFL4和PpnEPFL9相关。PpnMYC2与PagJAZs之间的互作是经过实验验证的。在杨树和拟南芥中过表达PpnMYC2基因会导致气孔密度降低。PpnMYC2过表达杨树具有更高的水分利用效率和干旱耐受性。转录组数据显示PpnMYC2过表达会导致气孔密度负调控因子PagEPF2和PagEPFL4的表达上调，而气孔密度正调控因子PagEPFL9的表达下调。作者通过酵母单杂、EMSA、ChIP-qPCR以及双荧光素酶试验验证了PpnMYC2能够直接调控PagEPF2、PagEPFL4和PagEPFL9的表达。另外，三倍体杨树气孔形成过程中，PpnMYC2、PpnEPF2和PpnEPFL4基因的表达显著上调，同时PpnEPFL9基因的表达显著下调。本文的研究结果揭示了植物气孔发生和多倍体气孔密度的调控机制，可以通过过表达MYC2基因来降低气孔密度和提升植物水分利用效率。
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个人简介：
1986年，内蒙古林学院，学士；
1986-1998年，甘肃农业大学，助教、讲师、副教授；
1991-1996年，北京林业大学，硕博连读；
1996-1998年，中国农业大学，博后；
1998年-至今，北京林业大学，副教授、教授。
研究方向：
1. 林木倍性育种理论与技术；
2. 林木良种选育；
3. 林木细胞遗传学；
4. 生物技术辅助育种。
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