周一“星”视角|基于高灵敏度检测平台的中国食管鳞癌诊断;18F-FAPI PET/CT预测新辅助卡瑞利珠单抗联合化疗的病理反应

学术   科学   2024-10-07 20:20   四川  



本期胸小星将为大家带来基于高灵敏度检测平台的中国食管鳞癌诊断;18F-FAPI PET/CT预测新辅助卡瑞利珠单抗联合化疗的病理反应,一起来看看吧!


2017·EATTS 

01

Highly sensitive detection platform-based diagnosis of Oesophageal squamous cell carcinoma in China: a multicentre, case–control, diagnostic study

Yu Wang1, Shan Xing2, Yi-Wei Xu3, Qing-Xia Xu4, Ming-Fang Ji5, Yu-Hui Peng6, Ya-Xian Wu7, Meng Wu8, Ning Xue9, Biao Zhang10, Shang-Hang Xie11, Rui-Dan Zhu12, Xin-Yuan Ou13, Qi Huang14, Bo-Yu Tian15, Hui-Lan Li16, Yu Jiang17, Xiao-Bin Yao18, Jian-Pei Li19, Li Ling20, Su-Mei Cao21, Qian Zhong22, Wan-Li Liu23, Mu-Sheng Zeng24

1 Department of Experimental Research, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, China.

2 Department of Clinical Laboratory, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, China.

3 Department of Clinical Laboratory Medicine, Cancer Hospital of Shantou University Medical College, Shantou, China.

4 Department of Clinical Laboratory, Affiliated Tumor Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou Key Laboratory of Digestive System Tumor Marker Diagnosis, Zhengzhou, China.

5 Cancer Research Institute of Zhongshan City, Zhongshan City People's Hospital, Zhongshan, China.

6 Department of Cancer Prevention Center, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, China.

7 The Second Clinical Faculty of Henan University of Chinese Medicine, Zhengzhou, China.

8 Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, China.

9 Department of Experimental Research, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, China. Electronic address: zengmsh@sysucc.org.cn.


Background: 

Early detection and screening of oesophageal squamous cell carcinoma rely on upper gastrointestinal endoscopy, which is not feasible for population-wide implementation. Tumour marker-based blood tests offer a potential alternative. However, the sensitivity of current clinical protein detection technologies is inadequate for identifying low-abundance circulating tumour biomarkers, leading to poor discrimination between individuals with and without cancer. We aimed to develop a highly sensitive blood test tool to improve detection of oesophageal squamous cell carcinoma.


Methods: 

We designed a detection platform named SENSORS and validated its effectiveness by comparing its performance in detecting the selected serological biomarkers MMP13 and SCC against ELISA and electrochemiluminescence immunoassay (ECLIA). We then developed a SENSORS-based oesophageal squamous cell carcinoma adjunct diagnostic system (with potential applications in screening and triage under clinical supervision) to classify individuals with oesophageal squamous cell carcinoma and healthy controls in a retrospective study including participants (cohort I) from Sun Yat-sen University Cancer Center (SYSUCC; Guangzhou, China), Henan Cancer Hospital (HNCH; Zhengzhou, China), and Cancer Hospital of Shantou University Medical College (CHSUMC; Shantou, China). The inclusion criteria were age 18 years or older, pathologically confirmed primary oesophageal squamous cell carcinoma, and no cancer treatments before serum sample collection. Participants without oesophageal-related diseases were recruited from the health examination department as the control group. The SENSORS-based diagnostic system is based on a multivariable logistic regression model that uses the detection values of SENSORS as the input and outputs a risk score for the predicted likelihood of oesophageal squamous cell carcinoma. We further evaluated the clinical utility of the system in an independent prospective multicentre study with different participants selected from the same three institutions. Patients with newly diagnosed oesophageal-related diseases without previous cancer treatment were enrolled. The inclusion criteria for healthy controls were no obvious abnormalities in routine blood and tumour marker tests, no oesophageal-associated diseases, and no history of cancer. Finally, we assessed whether classification could be improved by integrating machine-learning algorithms with the system, which combined baseline clinical characteristics, epidemiological risk factors, and serological tumour marker concentrations. Retrospective SYSUCC cohort I (randomly assigned [7:3] to a training set and an internal validation set) and three prospective validation sets (SYSUCC cohort II [internal validation], HNCH cohort II [external validation], and CHSUMC cohort II [external validation]) were used in this step. Six machine-learning algorithms were compared (the least absolute shrinkage and selector operator regression, ridge regression, random forest, logistic regression, support vector machine, and neural network), and the best-performing algorithm was chosen as the final prediction model. Performance of SENSORS and the SENSORS-based diagnostic system was primarily assessed using accuracy, sensitivity, specificity, and area under the receiver operating characteristic curve (AUC).


Results: 

Between Oct 1, 2017, and April 30, 2020, 1051 participants were included in the retrospective study. In the prospective diagnostic study, 924 participants were included from April 2, 2022, to Feb 2, 2023. Compared with ELISA (108.90 pg/mL) and ECLIA (41.79 pg/mL), SENSORS (243.03 fg/mL) showed 448 times and 172 times improvements, respectively. In the three retrospective validation sets, the SENSORS-based diagnostic system achieved AUCs of 0.95 (95% CI 0.90-0.99) in the SYSUCC internal validation set, 0.93 (0.89-0.97) in the HNCH external validation set, and 0.98 (0.97-1.00) in the CHSUMC external validation set, sensitivities of 87.1% (79.3-92.3), 98.6% (94.4-99.8), and 93.5% (88.1-96.7), and specificities of 88.9% (75.2-95.8), 74.6% (61.3-84.6), and 92.1% (81.7-97.0), respectively, successfully distinguishing between patients with oesophageal squamous cell carcinoma and healthy controls. Additionally, in three prospective validation cohorts, it yielded sensitivities of 90.9% (95% CI 86.1-94.2) for SYSUCC, 84.8% (76.1-90.8) for HNCH, and 95.2% (85.6-98.7) for CHSUMC. Of the six machine-learning algorithms compared, the random forest model showed the best performance. A feature selection step identified five features to have the highest performance to predictions (SCC, age, MMP13, CEA, and NSE) and a simplified random forest model using these five features further improved classification, achieving sensitivities of 98.2% (95% CI 93.2-99.7) in the internal validation set from retrospective SYSUCC cohort I, 94.1% (89.9-96.7) in SYSUCC prospective cohort II, 88.6% (80.5-93.7) in HNCH prospective cohort II, and 98.4% (90.2-99.9) in CHSUMC prospective cohort II. 


Conclusions: 

The SENSORS system facilitates highly sensitive detection of oesophageal squamous cell carcinoma tumour biomarkers, overcoming the limitations of detecting low-abundance circulating proteins, and could substantially improve oesophageal squamous cell carcinoma diagnostics. This method could act as a minimally invasive screening tool, potentially reducing the need for unnecessary endoscopies.


[CITATION]: Yu Wang, Shan Xing, Yi-Wei Xu, et al. Highly sensitive detection platform-based diagnosis of Oesophageal squamous cell carcinoma in China: a multicentre, case–control, diagnostic study. Lancet Digit Health. 2024 Oct;6(10): e705-e717.

[DOI]: 10.1016/S2589-7500(24)00153-5.

[IF]: 23.8

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基于高灵敏度检测平台的中国食管鳞状细胞癌诊断:一项多中心病例对照诊断研究

胸“星”外科学术团队成员 杨凯越 

背景

食管鳞状细胞癌的早期发现和筛查依赖于上消化道内镜,但这并不适用于所有人群。基于肿瘤标志物的血液检测是一种潜在的替代方法。然而,目前临床蛋白检测技术的灵敏度不足以识别低丰度循环肿瘤生物标志物,导致对癌症患者和非癌症患者的区分度较低。本研究旨在开发一种高灵敏度的血液检测工具,以提高食管鳞状细胞癌的检测率。

方法

本研究设计了一个名为SENSORS的检测平台。通过比较其与酶联免疫吸附实验(Enzyme linked immunosorbent assay,ELISA)和电化学发光免疫分析法(Electrochemiluminescence immunoassay,ECLIA)在检测选定血清学生物标志物MMP13和SCC方面的性能,验证了其有效性。随后,本研究开发了基于SENSORS的食管鳞状细胞癌辅助诊断系统(可应用于临床监督下的筛查和分诊),对一项回顾性研究中的食管鳞状细胞癌患者和健康对照者进行分类。研究对象包括中山大学肿瘤防治中心(Sun Yat-sen University Cancer Center, SYSUCC,中国广州)、河南省肿瘤医院(Henan Cancer Hospital, HNCH;中国郑州)和汕头大学医学院肿瘤医院(Cancer Hospital of Shantou University Medical College, CHSUMC;中国汕头)的参与者(队列I)。纳入标准为:年龄≥18岁,病理证实为原发性食管鳞状细胞癌,且在血清标本采集前未接受过肿瘤治疗。对照组为从健康检查部门招募的没有食道相关疾病的参与者。基于SENSORS的诊断系统以多因素逻辑回归模型为基础,该模型将SENSORS的检测值作为输入值,并输出预测食管鳞状细胞癌可能性的风险评分。本研究在一项独立的前瞻性多中心研究中进一步评估了该系统的临床效用。从上述三个机构中筛选出不同的参与者,并招募了新诊断为食管相关疾病且既往未接受过癌症治疗的患者。健康对照组的纳入标准为血常规和肿瘤标志物检查无明显异常,无食道相关疾病,无癌症史。最后,结合基线临床特征、流行病学风险因素和血清学肿瘤标记物浓度,评估了是否可以通过将机器学习算法与系统相结合来改进分类。在此步骤中使用了回顾性SYSUCC队列I(随机分配[7:3]到训练集和内部验证集)和三个前瞻性验证集(SYSUCC队列II[内部验证]、HNCH队列II[外部验证]和CHSUMC队列II[外部验证])。比较了六种机器学习算法(最小绝对收缩和选择算子回归、脊回归、随机森林、逻辑回归、支持向量机和神经网络),并将表现最好的算法作为最终的预测模型。主要通过准确性、灵敏度、特异性和受者工作特征曲线下面积(AUC),对SENSORS和基于SENSORS的诊断系统的性能进行评估。

结果

在2017年10月1日至2020年4月30日期间,有1051名参与者参与了回顾性研究。在前瞻性诊断研究中,纳入了从2022年4月2日至2023年2月2日的924名参与者。与ELISA(108.90 pg/mL)和ECLIA(41.79 pg/mL)相比,SENSORS(243.03 fg/mL)分别提高了448倍和172倍。在三个回顾性验证集中,基于SENSORS的诊断系统在SYSUCC内部验证集中的AUC值为0.95 (95% CI:0.90 - 0.99),在HNCH外部验证集中的AUC值为0.93 (95% CI:0.89 - 0.97),在CHSUMC外部验证集中的AUC值为0.98 (95% CI:0.97 - 1.00),灵敏度分别为87.1%(79.3 - 92.3)、98.6%(94.4 - 99.8)和93.5%(81.1 - 96.7),特异性分别为88.9%(75.2 - 95.8)、74.6%(61.3 - 84.6)和92.1%(81.7 - 97.0),成功区分了食管鳞状细胞癌患者和健康对照组。此外,在三个前瞻性验证队列中,SYSUCC的灵敏度为90.9% (95% CI:86.1  -  94.2),HNCH的灵敏度为88.4 %(76.1  -  98.9),CHSUMC的灵敏度为95.2 %(85.6  -  98.7)。在比较的六种机器学习算法中,随机森林模型的表现最好。通过特征选择步骤,确定了预测效果最好的五个特征(SCC、年龄、MMP13、CEA和NSE),使用这五个特征的简化随机森林模型进一步提高了分类效果。在回顾性SYSUCC队列I的内部验证集中,灵敏度为98.2% (95% CI:93.2  -  99.7),SYSUCC前瞻性队列II中的灵敏度为94.1% (89.9  -  99.7),在HNCH前瞻性队列II中的灵敏度为88.6% (85.5  -  93.7);在CHSUMC前瞻性队列II中的灵敏度为98.4% (99.2  -  99.9)。

结论

SENSORS系统实现了食管鳞状细胞癌肿瘤生物标志物的高灵敏度检测,克服了低丰度循环蛋白检测的局限性,可大幅提高食管鳞状细胞癌的诊断水平。这种方法可作为一种微创筛查工具,潜在地减少不必要的内镜检查。

Table 1. Diagnostic performance of SENSORS for detecting the SCC and MMP13 protein in comparison with ECLIA and ELISA.


Figure 2. Performance evaluation of the SENSORS-based diagnostic system in discriminating patients with oesophageal squamous cell carcinoma from healthy controls in a retrospective multicentre study.

Table 2. Performance of the SENSORS-based diagnostic system in discriminating patients with oesophageal squamous cell carcinoma from healthy controls in a retrospective multicentre cohort.


Table 3. Performance of the SENSORS-based diagnostic system in discriminating patients with oesophageal squamous cell carcinoma and those with oesophageal cancer from healthy controls in a prospective multicentre cohort.

2017·EATTS 

02

[18F]AlF-NOTA-FAPI-04 PET/CT for Predicting Pathologic Response of Resectable Esophageal Squamous Cell Carcinoma to Neoadjuvant Camrelizumab and Chemotherapy: A Phase II Clinical Trial

Yinjun Dong1 , Zhendan Wang2 , Xinying Hu3 , Yuhong Sun4 , Jingjie Qin3 , Qiming Qin1 , Shuguang Liu1 , Shuanghu Yuan3,5, Jinming Yu3 , and Yuchun Wei3 

1 Department of Esophageal Surgery, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

2 Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

3 Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

4 Department of Pathology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

5 Department of Radiation Oncology, Division of Life Sciences and Medicine, First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China.


Background: 

This single-center, single-arm, phase II trial (ChiCTR2100050057) investigated the ability of 18F-labeled fibroblast activation protein inhibitor ([18F]AlF-NOTA-FAPI-04, denoted as 18F-FAPI) PET/CT to predict the response to neoadjuvant camrelizumab plus chemotherapy (nCC) in locally advanced esophageal squamous cell carcinoma (LA-ESCC). 


Methods: 

This study included 32 newly diagnosed LA-ESCC participants who underwent 18F-FAPI PET/CT at baseline, of whom 23 also underwent scanning after 2 cycles of nCC. The participants underwent surgery after 2 cycles of nCC. Recorded PET parameters included maximum, peak, and mean SUVs and tumor-to-background ratios (TBRs), metabolic tumor volume, and total lesion FAP expression. PET parameters were compared between patient groups with good and poor pathologic responses, and the predictive performance for treatment response was analyzed.


Results: 

The good and poor response groups each included 16 participants (16/32, 50.0%). On 18F-FAPI PET/CT, the posttreatment SUVs were significantly lower in good responders than in poor responders, whereas the changes in SUVs with treatment were significantly higher (all P < 0.05). SUVmax (area under the curve [AUC], 0.87; P = 0.0026), SUVpeak (AUC, 0.89; P = 0.0017), SUVmean (AUC, 0.88; P = 0.0021), TBRmax (AUC, 0.86; P = 0.0031), and TBRmean (AUC, 0.88; P = 0.0021) after nCC were significant predictors of pathologic response to nCC, with sensitivities of 63.64%–81.82% and specificities of 83.33%–100%. Changes in SUVmax (AUC, 0.81; P = 0.0116), SUVpeak (AUC, 0.82; P = 0.0097), SUVmean (AUC, 0.81; P = 0.0116), and TBRmean (AUC, 0.74; P = 0.0489) also were significant predictors of the pathologic response to nCC, with sensitivities and specificities in similar ranges.


Conclusion: 

18F-FAPI PET/CT parameters after treatment and their changes from baseline can predict the pathologic response to nCC in LA-ESCC participants.


[CITATION]: Dong Y, Wang Z, Hu X, et al. [18F]AlF-NOTA-FAPI-04 PET/CT for Predicting Pathologic Response of Resectable Esophageal Squamous Cell Carcinoma to Neoadjuvant Camrelizumab and Chemotherapy: A Phase II Clinical Trial. J Nucl Med. 2024 Sep 26:jnumed.124.268557. 

[DOI]: 10.2967/jnumed.124.268557.

[IF]:9.1

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[18F]AlF-NOTA-FAPI-04 PET/CT预测新辅助卡瑞利珠单抗联合化疗对可切除食管鳞状细胞癌的病理反应:一项II期临床试验

胸“星”外科学术团队成员 周灵 译


目的

本研究是一项单中心、单臂、II期临床试验(ChiCTR2100050057),旨在研究18F标记的成纤维细胞激活蛋白抑制剂([18F]AlF-NOTA-FAPI-04,简称18F-FAPI)PET/CT预测局部晚期食管鳞状细胞癌(locally advanced esophageal squamous cell carcinoma,LA-ESCC)患者对新辅助卡瑞利珠单抗联合化疗(neoadjuvant camrelizumab plus chemotherapy,nCC)反应的能力。

方法

本研究共纳入32名新诊断的LA-ESCC患者,他们在基线时接受了18F-FAPI PET/CT检查,其中23名在2个周期的nCC后再次进行检查。患者在2个周期的nCC后进行手术。本研究记录的PET参数包括最大、峰值和平均标准摄取值、肿瘤与背景比(tumor-to-background ratios ,TBRs)、代谢肿瘤体积和总病变FAP表达。比较了病理反应良好组和不良组的患者的PET参数,并分析了治疗后病理反应的PET参数预测性能。

结果

良好和不良组各有16名患者(16/32,50%)。18F-FAPI PET/CT显示,良好组的治疗后SUVs显著低于不良组,但SUVs的变化则显著高于不良组(均P < 0.05)。nCC后SUVmax(曲线下面积(area under the curve,AUC): 0.87;P = 0.0026)、SUVpeak(AUC: 0.89;P = 0.0017)、SUVmean(AUC: 0.88;P = 0.0021)、TBRmax(AUC: 0.86;P = 0.0031)和TBRmean(AUC: 0.88;P = 0.0021)是nCC病理反应的重要预测因素,敏感性为63.64%-81.82%,特异性为83.33%-100%。SUVmax(AUC: 0.81;P = 0.0116)、SUVpeak(AUC: 0.82;P = 0.0097)、SUVmean(AUC: 0.81;P = 0.0116)和TBRmean(AUC: 0.74;P = 0.0489)的变化也是nCC病理反应的重要预测因素,敏感性和特异性在相似范围内。

结论

治疗后的18F-FAPI PET/CT参数及其基线值的变化可以预测LA-ESCC患者对nCC的病理反应。

Table 3. Comparison of 18F-FAPI PET/CT Parameters at Different Time Points Between Patient Groups with Good and Poor Responses to nCC.


Figure 4. Receiver operating characteristic curves for assessing predictive accuracy of SUVs on 18F-FAPI PET/CT for identifying good and poor pathologic responders to nCC among participants with LA-ESCC. 

2017·EATTS 



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