摘要来源：journals.sagepub.com/doi/full/10.1177/1098612X241241408
一校：郭嘉恩
二校：kenda
摘要
目标
方法
结果
在IgG EIA检测中，五只猫呈阳性（5/8，诊断敏感性为62.5%；95%置信区间 [CI] 24.5–91.5），在LFA检测中，五只猫呈阳性（5/7，诊断敏感性为71.4%；95% CI 29.0–96.3）。结合两种检测方法进行诊断的敏感性为87.5%（7/8，95% CI 47.3–99.7）。IgG EIA的荚膜组织胞浆菌抗体检测特异性为100%（95% CI 71.5–100），LFA的荚膜组织胞浆菌抗原检测特异性也为100%（95% CI 71.5–100）。
结论和相关性
使用半定量间接IgG抗体EIA检测血清中的荚膜组织胞浆菌抗体，和使用LFA检测尿液中的荚膜组织胞浆菌抗原，是新的微创诊断技术，有助于诊断猫弥散性和肺部组织胞浆菌病，尤其是同时结合两种技术时，准确率更高。
原文：
Histoplasmosis in domestic cats: new minimally invasive diagnostic techniques
https://doi.org/10.1177/1098612X241248984
Abstract
Objectives
The aim of the present study was to evaluate minimally invasive diagnostic techniques, such as the semi-quantitative indirect IgG antibody enzyme immunoassay (EIA) using blood serum and the urinary lateral flow assay (LFA), for the detection of Histoplasma capsulatum in cats with histoplasmosis.
Methods
Eight client-owned domestic cats diagnosed with histoplasmosis were selected based on cytological, histopathological, mycological, molecular or antigenic techniques. The blood serum of these animals was tested in a semi-quantitative indirect IgG antibody EIA for the detection of H capsulatum. Urine samples were tested for H capsulatum antigen using LFA.
Results
Five cats were seropositive on IgG EIA (5/8, with diagnostic sensitivity equal to 62.5%; 95% confidence interval [CI] 24.5–91.5) and five cats were positive on H capsulatum antigen LFA (5/7, with diagnostic sensitivity equal to 71.4%; 95% CI 29.0–96.3). The combined diagnostic sensitivity when interpreted in parallel was 87.5% (7/8, 95% CI 47.3–99.7). The specificity for the anti-Histoplasma IgG EIA was 100% (95% CI 71.5–100) and for the H capsulatum antigen LFA it was also 100% (95% CI 71.5–100).
Conclusions and relevance
The semi-quantitative indirect IgG antibody EIA for the detection of H capsulatum in blood serum and the urinary LFA for the detection of the same agent emerge as new minimally invasive diagnostic techniques that can assist in the approach to disseminated and pulmonary feline histoplasmosis, especially when both techniques are considered together.
