The backdrop features a grafted chrysanthemum, symbolizing the focus of our research. Its graft-junction, accentuated by a light halo, underscores the intricate process of grafting. Zooming in, a leaf is depicted adorned with numerous 'sentry towers,' each representing glandular trichomes. One such tower under construction signifies ongoing biological processes, receiving vital elements-represented as particles (JA) flying in from the grafted chrysanthemum-contributing to its growth. A compelling narrative unfolds as these sentry towers launch arrows (terpenes) in defense against the invading Alternaria alternata fungus, illustrating nature's intricate defense mechanisms. See Li et al., pp. 6523–6541.
PLANT PHYSIOLOGY:西北农林发现一个介导ABA和生长素相互拮抗的转录因子
过表达ABA合成及信号转导相关基因随会提高植物对干旱胁迫的抗性,但会导致植物生长发育受到抑制。作者前期证实一个bZIP transcription factor TaFD-Like2-1A (TaFDL2-1A)可以通过激活ABA信号提高植物干旱胁迫的耐受性,但是会导致植物生长发育受到严重抑制。
本研究发现,过表达 TaFDL2-1A会降低细胞长度和宽度,对应的叶片尺寸及地上部的长度和干物质均显著下降。通过转录组分析发现,TaFDL2-1影响了生长素信号转导途径中基因的表达。进一步的分析显示,在TaFDL2-1A的过表达株系中Gretchen Hagen3 (GH3) 的表达受到诱导,同时生长素的水平显著下降。外施生长素可以恢复TaFDL2-1A过表达株系发育受阻的表型。
转录分析显示,TaFDL2-1A 诱导了包括TaGH3.2-3A, TaGH3.2-3B, TaGH3.8-2A, and TaGH3.8-2D在内的生长素代谢相关基因的表达, 它可以直接结合到上述基因启动子的ACGT core cis-elements上. 而tafdl2 knock-out株系中GH3基因的表达被抑制,同时生长素水平显著提高。作者证实,ABA是通过抑制生长素水平导致TaFDL2-1A过表达株系发育受阻。
原文:The transcription factor TaFDL2-1A functions in auxin metabolism mediated by abscisic acid to regulate shoot growth in wheat