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Engineering the complexity and controllability of human neural organoids
Human neural organoids generated from stem cells are powerful experimental tools for studying neural development and the pathogenesis of neurodevelopmental disorders. Despite considerable progress, it remains challenging to achieve high efficiency, controllability, and in vivo-like cellular organization and tissue architecture in current organoids. Here, I present a microfluidic platform that precisely control the morphology and morphogen environments of human stem cells to mimic the neural tube environment in vivo. Using this platform, we developed a completely patterned neural tube organoid that contains both brain- and spinal cord-like regions, offering a more in vivo-relevant model for human development and diseases.
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