离子组与砷形态分析为亚砷酸盐氧化菌协同磷酸盐缓解水稻中亚砷酸盐毒性提供证据
Ionome profiling and arsenic speciation provide evidence of arsenite detoxification in rice by phosphate and arsenite-oxidizing bacteria
Guobing Lin, Xiaoman He, Jiayuan Zeng, Zhaoguang Yang, Lin Wang*
https://doi.org/10.1016/j.jes.2022.08.017
摘要
稻田中的砷以生物毒性和流动性最强的亚砷酸盐(As(III))为主要形态，导致水稻中砷的积累量高于其他陆生作物。缓解砷对水稻的毒性是保障粮食生产和安全的重要途径。本研究通过在水稻生长体系中接种As(III)氧化细菌假单胞菌株SMS11的方式，实现As(III)向低毒性砷酸盐(As(V))的快速转化。同时，通过补充磷(P)来限制水稻对As(V)的吸收。外界As(III)胁迫显著抑制了水稻植株的生长，而通过添加P和SMS11可有效缓解As(III)的抑制作用。砷形态分析表明，P通过与As(V)竞争吸收途径限制水稻根系中砷的积累，而SMS11进一步限制了砷从水稻根系向茎叶的转运。离子组学分析揭示了不同处理组水稻组织样品的离子特征。与根系相比，水稻茎叶的离子组对外界环境扰动更为敏感。添加的P和As(III)氧化菌SMS11均可通过促进水稻生长和调节离子平衡来缓解As(III)胁迫。
亮点
从离子组学角度分析水稻响应外界As(III)胁迫的机制。As(III)氧化菌与P协同作用，通过转化砷形态与调节水稻离子平衡的方式缓解As(III)对水稻的毒性，抑制砷向水稻地上部迁移。
Abstract
Arsenite (As(III)) as the most toxic and mobile form is the dominant arsenic (As) species in flooded paddy fields, resulting in higher accumulation of As in paddy rice than other terrestrial crops. Mitigation of As toxicity to rice plant is an important way to safeguard food production and safety. In the current study, As(III)-oxidizing bacteria Pseudomonas sp. strain SMS11 was inoculated with rice plants to accelerate conversion of As(III) into lower toxic arsenate (As(V)). Meanwhile, additional phosphate was supplemented to restrict As(V) uptake by the rice plants. Growth of rice plant was significantly inhibited under As(III) stress. The inhibition was alleviated by the introduction of additional P and SMS11. Arsenic speciation showed that additional P restricted As accumulation in the rice roots via competing common uptake pathways, while inoculation with SMS11 limited As translocation from root to shoot. Ionomic profiling revealed specific characteristics of the rice tissue samples from different treatment groups. Compared to the roots, ionomes of the rice shoots were more sensitive to environmental perturbations. Both extraneous P and As(III)-oxidizing bacteria SMS11 could alleviate As(III) stress to the rice plants through promoting growth and regulating ionome homeostasis.
作者简介
第一作者
林国冰 于2023年在中南大学化学化工学院获得硕士学位，目前在浙江大学攻读博士学位，主要从事环境砷污染治理与修复方面的研究。
通讯作者
王琳 现任中南大学化学化工学院副教授、博士生导师。主要从事废水处理、重金属污染修复、微生物修复技术与食品安全相关的研究方向。主持承担包括国家自然科学、湖南省自然科学基金、国家重点研发计划等在内的多个项目/课题。以通讯作者在国内外主要期刊发表SCI论文30余篇，谷歌学术的H指数16。
原文链接
https://www.sciencedirect.com/science/article/pii/S1001074222004193
引用格式
Guobing Lin, Xiaoman He, Jiayuan Zeng, Zhaoguang Yang, Lin Wang, 2023. Ionome profiling and arsenic speciation provide evidence of arsenite detoxification in rice by phosphate and arsenite-oxidizing bacteria. J. Environ. Sci. 128, 129-138.
