慢性伤口遭遇多药耐药菌感染是致命的，严重者会引发脓毒症。抗菌肽的广谱抗菌特性和良好的抗耐药性被广泛用于伤口护理领域。
近日，青岛大学于冰教授和丛海林教授等人在Science China Materials发表研究论文，以SiO2微球为固定相，提取细菌的细胞膜制备细菌细胞膜色谱（BCMC）。
本文要点
1) 固相法合成抗菌肽文库并经BCMC筛选出抗菌肽LKAHR（简称LS5）。LS5具有生物安全、广谱抗菌以及抗耐药性等特点，制备明胶基抗菌水凝胶（LS5-gel）以更好应用于伤口。2) 在伤口愈合模型中发现LS5-gel具有良好的体内杀菌性能以及促进伤口愈合能力。在脓毒症模型中发现LS5对脓毒症感染具有明显的抑制作用。本工作对下一代抗菌药物的选取及慢性伤口愈合治疗具有重要指导意义。Figure 1. LS5 screening and LS5-gel mechanism diagram. (a) Schematic of membrane chromatography preparation and LS5 screening. (b) Schematic diagram of antibacterial hydrogel LS5-gel preparation. (c) LS5-gel and LS5 in the treatment of wound infections and sepsis.Figure 2. Preparation and characterization of hydrogels. (a) SEM images of hydrogels and antimicrobial peptide-carrying hydrogels. (b) IR spectral analysis of gelatin gels and LS5 hydrogels. (c) Rheological test of GT-gel and LS5-gel. (d) LS5 hydrogel inhibition circle experiment. (e) Degradation rate test of GT-gel and LS5-gel. (f) Swelling rate test of LS5-gel.Figure 3. Stability and resistance testing of LS5. (a) Bacterial coatings for LS5 resistance testing. (b) Comparison of MIC curves of LS5 and imipenem against S. aureus. (c) Comparison of MIC curves of LS5 and imipenem against E. coli. (d) Acid–base stability of LS5. (e) Measurement of thermal stability of LS5. (f) Stability of LS5 in simulated artificial gastric fluid. (g) Measurement of ionic stability of LS5.Figure 4. Mouse wound healing model. (a) Schematic diagram of the experimental model. (b) Wound healing pictures and analog digital pictures of LS5 and LS5-gel over 12 days. (c) H&E and Masson sections of wound healing in each group. (d) Statistics on the change of wound area in each group of mice. (e) Body weight monitoring data for each group of mice. (f) Epidermal thickness index. (g) Dermal thickness index. (h) Wound collagen accumulation area statistics. *p < 0.05, **p < 0.05, ***p < 0.05.Zekai Ren, Xin Ding, Yumei Wang, Han Wu, Xin Liu, Yang Cao, Hailin Cong, Youqing Shen, Bing Yu. A pro-healing short-chain antimicrobial peptide that inhibits sepsis. Sci. China Mater. (2024).https://doi.org/10.1007/s40843-024-3002-0
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