近日，华南农大曹必好-颜爽爽团队在Plant Cell Environment发表了题为《SmHSFA8 Enhances the Heat Tolerance of Eggplant by Regulating the SmEGY3-SmCSD1 Module and Promoting SmF3H-mediated Flavonoid Biosynthesis》的研究论文，揭示了HSFA8通过调节EGY3-CSD1模块并促进F3H介导的黄酮生物合成来增强茄子耐热性。
高温（HT）是抑制茄子生长和产量的主要环境因子。热激因子（HSFs）在植物对高温胁迫（HTS）的响应中起着至关重要的作用。然而，HSFs调节茄子耐热性的分子机制尚不清楚。
作者先前的研究报道了SmEGY3增强了茄子的耐热性。在本研究中，作者进一步报道，SmHSFA8激活了SmEGY3的表达，并与SmEGY3蛋白相互作用以增强SmEGY3对SmCSD1的激活功能。
病毒诱导的基因沉默（VIGS）和过表达实验表明，SmHSFA8正向调节植物的耐热性。SmHSFA8通过促进SlEGY3表达、H2O2产生和H2O2介导的反向信号传导途径来增强番茄植株的耐热性。DNA亲和纯化测序（DAP-seq）分析揭示了SmHSPs（SmHSP70、SmHSP70B和SmHSP21）和SmF3H是SmHSFA8的可能下游靶基因。
SmHSFA8调节了植物中的HSPs和F3H以及黄酮含量。通过VIGS沉默SmF3H降低了茄子的黄酮含量和耐热性。此外，外源黄酮处理缓解了高温胁迫对茄子的损害。
这些结果表明，SmHSFA8通过激活SmHSPs表达、介导SmEGY3-SmCSD1模块以及促进SmF3H介导的黄酮生物合成来增强茄子的耐热性。
High temperature (HT) is a major environmental factor that restrains eggplant growth and production. Heat shock factors (HSFs) play a vital role in the response of plants to high-temperature stress (HTS). However, the molecular mechanism by which HSFs regulate heat tolerance in eggplants remains unclear. Previously, we reported that SmEGY3 enhanced the heat tolerance of eggplant. Herein, SmHSFA8 activated SmEGY3 expression and interacted with SmEGY3 protein to enhance the activation function of SmEGY3 on SmCSD1. Virus-induced gene silencing (VIGS) and overexpression assays suggested that SmHSFA8 positively regulated heat tolerance in plants. SmHSFA8 enhanced the heat tolerance of tomato plants by promoting SlEGY3 expression, H2O2 production and H2O2-mediated retrograde signalling pathway. DNA affinity purification sequencing (DAP-seq) analysis revealed that SmHSPs (SmHSP70, SmHSP70B and SmHSP21) and SmF3H were candidate downstream target genes of SmHSFA8. SmHSFA8 regulated the expression of HSPs and F3H and flavonoid content in plants. The silencing of SmF3H by VIGS reduced the flavonoid content and heat tolerance of eggplant. In addition, exogenous flavonoid treatment alleviated the HTS damage to eggplants. These results indicated that SmHSFA8 enhanced the heat tolerance of eggplant by activating SmHSPs exprerssion, mediating the SmEGY3-SmCSD1 module, and promoting SmF3H-mediated flavonoid biosynthesis.
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