材料方法（Materials & Methods）的写作难度，在整篇论文中应该算是最好写的了。但实际情况是，很多论文在这块仍然存在不少问题。要想论文得到认可，方法部分必须让人一目了然又专业。接下来，跟着顶刊一起来学习吧。
一 Methods写作原则
二 撰写Methods容易出现哪些问题呢
三 CNS文章材料方法拆解
案例拆解：来自Cell 期刊
图片来源：https://mitcommlab.mit.edu/be/commkit/journal-article-methods/
四 Materials & Methods句型模板
最后，部分句型模板供参考：
1. 描述材料和设备
● We utilized [specific equipment/model] (manufacturer, country) to conduct [specific procedure].
● [Sample/material] was prepared by [method] under [specific conditions].
● All chemicals/reagents were obtained from [supplier], with a purity of [purity level].
2. 详细描述实验步骤
● Each [sample/specimen] was [processed/prepared] by [method, e.g., ‘incubating’] at [temperature] for [duration].
● [Step/Procedure] was repeated [number of times] to ensure [purpose, e.g., consistency, accuracy].
● To [achieve specific goal], [procedure] was performed as follows: [describe step-by-step].
3. 数据收集和分析方法
● Data were collected using [instrument/software version] with parameters set at [specific values].
● Analysis was conducted using [statistical software/method] at a significance level of [p-value threshold].
● For statistical comparison, we applied [method, e.g., ‘ANOVA’, ‘t-test’], with adjustments for [variables, if applicable].
4. 提供实验依据或特殊条件
● To account for [variable or experimental condition], we modified [method] by [specific change].
● This method was selected due to its ability to [reason, e.g., ‘maximize accuracy in...’].
● Given [specific limitation or challenge], we implemented [additional step/alternative method].
5. 描述对照组和重复实验
● Control groups were established by [describe control setup], ensuring consistency across [variables].
● Each experiment was performed in triplicate to enhance reproducibility and statistical reliability.
● Replicates were conducted to minimize variation, with [number] independent trials for each condition.
欢迎添加咨询或备用哦~
[1]https://www.researchgate.net/publication/373507580_How_to_Write_the_Methods_Section_of_a_Research_Manuscript
[2]https://mitcommlab.mit.edu/be/commkit/journal-article-methods/
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感觉不错，点个和在看呗~
