图1. GhMYB30D04激活GhLTPG1启动子以及Ghmyb30d04突变体的相关表型
蛋白互作筛选发现，GhMYB30D04与GhHD1能够形成的转录复合体，增强对GhLTPG1启动子的激活活性和结合强度，两者的相互作用依赖于GhHD1的START-SAD结构域和HD结构域。但是，GhHD1和GhMYB30D04相互之间并不存在转录调控。GhHD1蛋白可以结合多种磷脂酰肌醇，其保守的磷脂结合位点突变后会改变其核定位，导致其不能与GhMYB30D04在核中形成转录复合体而增强对GhLTPG1的激活。同时，GhHD1与GhMYB30D04在棉花中共同调控GhLTPG1的表达水平和纤维细胞的伸长发育 (图2)。
图2. GhHD1-GhMYB30D04复合物激活GhLTPG1表达以维持棉纤维伸长过程中PIP含量的工作模型
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