Cancer Cell Int
2021 Oct 18;21(1):550.
doi: 10.1186/s12935-021-02253-8.
LncRNA LINC00337 sponges mir-1285-3p to promote proliferation and metastasis of lung adenocarcinoma cells by upregulating YTHDF1
Ru-Nan Zhang 1, Dong-Mei Wu 2, Li-Ping Wu 2, Guo-Wei Gao 2
Affiliations expand
PMID: 34663343
PMCID: PMC8524958
DOI: 10.1186/s12935-021-02253-8
Retraction in
Retraction Note: LncRNA LINC00337 sponges mir-1285-3p to promote proliferation and metastasis of lung adenocarcinoma cells by upregulating YTHDF1.
Zhang RN, Wu DM, Wu LP, Gao GW.Cancer Cell Int. 2024 Jul 14;24(1):245. doi: 10.1186/s12935-024-03431-0.PMID: 39004724 Free PMC article. No abstract available.
Abstract
Background: Emerging studies have shown that long noncoding RNAs (lncRNAs) predominantly function in the carcinogenesis of multiple developing human tumors. The current study aimed to investigate the underlying mechanisms of LINC00337 in lung adenocarcinoma.
Methods: We analyzed TCGA and GTEx datasets and chose LINC00337 as the research object. Cell proliferation, cell apoptosis, cell cycle, migration, and invasion were detected in the gain and loss experiments of LINC00337 both in vitro and in vivo. Moreover, RNA pull-down, luciferase reporter assays, western blotting analysis, and rescue experiments were performed to investigate the underlying molecular mechanisms of LINC00337 function.
Results: LINC00337 expression was remarkably upregulated in lung adenocarcinoma. In addition, LINC00337 knockdown was shown to repress cell migration, invasion, and proliferation, as well as the cell cycle, and gear up apoptosis in lung adenocarcinoma in vitro and in vivo. With respect to the mechanism, LINC00337 knockdown boosted miR-1285-3p expression and then restrained YTHDF1 expression post-transcriptionally. Crucially, both miR-1285-3p decrement and YTHDF1 overexpression successfully reversed the influence on cell proliferation, migration, invasion, and apoptosis caused by LINC00337 shRNA.
Conclusions: These results suggest that LINC00337 acts as an oncogenic lncRNA, targeting miR-1285-3p and regulating YTHDF1 expression, to promote the progression of lung adenocarcinoma.
Keywords: Cell invasion; Cell proliferation; LINC00337; Lung adenocarcinoma; lncRNA.
参考链接：https://pubpeer.com/publications/B4EBFB16EAB885A7E99C83FC36D241
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