Autophagy
2019 Apr;15(4):668-685.
doi: 10.1080/15548627.2018.1539590. Epub 2018 Nov 5.
GRSF1-mediated MIR-G-1 promotes malignant behavior and nuclear autophagy by directly upregulating TMED5 and LMNB1 in cervical cancer cells
Zhen Yang 1, Qi Sun 1, Junfei Guo 1, Shixing Wang 1, Ge Song 1, Weiying Liu 1, Min Liu 1, Hua Tang 1
Affiliations expand
PMID: 30394198
PMCID: PMC6526811
DOI: 10.1080/15548627.2018.1539590
Erratum in
Correction.
[No authors listed]Autophagy. 2021 Nov;17(11):3889-3892. doi: 10.1080/15548627.2021.1874198. Epub 2021 Jan 18.PMID: 33461380 Free PMC article. No abstract available.
Abstract
Emerging evidence has revealed that miRNAs could upregulate the expression levels of target genes. However, the molecular mechanism underlying upregulation of targets mediated by miRNAs remains unclear. In this study, we found a novel miRNA named MIR-G-1 by GRSF1-RNA immunoprecipitation (RIP)-deep sequencing, which could directly target and upregulate LMNB1 and TMED5 in a GRSF1-dependent manner in cervical cancer cells. In addition, upregulated MIR-G-1 in cervical cancer promoted a malignant phenotype in vitro and in vivo. TMED5 could interact with WNT7B and thus activated the canonical WNT-CTNNB1/β-catenin signaling pathway. MIR-G-1 mediated the activation of this pathway. Furthermore, MIR-G-1 promoted serum starvation-induced nuclear macroautophagy/autophagy, and accelerated taxol (TAX)-induced DNA-damage repair in cervical cancer cells. Collectively, these findings may provide a new insight into the upregulation mechanism and nuclear autophagy mediated by miRNAs and provide a potential biomarker for cervical cancer. Abbreviations: 3'UTR: 3' untranslated region; EMSA: electrophoretic mobility shift assay; EMT: epithelial-mesenchymal transition; GRSF1: G-rich RNA sequence binding factor 1; IF: immunofluorescence; IP: immunoprecipitation; IHC: immunohistochemistry; lnc: long noncoding; miRNA:microRNA; TAX: taxol; TMED5: transmembrane p24 trafficking protein 5.
Keywords: Cervical cancer; GRSF1; LMNB1; TMED5; novel miRNA; nuclear autophagy.
参考连接：
https://pubpeer.com/publications/08A4F12C1332E0B7DC24158CE5D76C
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