Biomed Pharmacother
2018 May:101:406-413.
doi: 10.1016/j.biopha.2018.02.104. Epub 2018 Mar 22.
miR-615-3p promotes proliferation and migration and inhibits apoptosis through its potential target CELF2 in gastric cancer
Jizhao Wang 1, Lin Liu 1, Yuchen Sun 2, Yumo Xue 3, Jingkun Qu 1, Shupei Pan 2, Huajing Li 1, Hangying Qu 1, Jiansheng Wang 4, Jia Zhang 5
Affiliations expand
PMID: 29501762
DOI: 10.1016/j.biopha.2018.02.104
Free article
Abstract
Gastric cancer incidence is relatively higher in China than that in developed countries; however, molecular mechanisms considering the initiation and progression of gastric cancer are still unclear. For decades, numerous microRNAs have been found to regulate a wide range of biological functions in gastric cancer. However, the oncogenic function of miR-615-3p in gastric cancer has not been reported to date. With the help of gene and microRNA chips in 10 patients, we were able to screen differential expressed genes and microRNAs compared with normal gastric tissues. After that, online bioinformatics analysis tools were used to predict microRNAs' potential targets. As a result, miR-615-3p and its potential target, CELF2, were selected for further experiments. QRT-PCR and western blot results indicated the aberrant high expression of miR-615-3p and low expression of CELF2 in gastric cancer both in vivo and in vitro. Moreover, miR-615-3p expression correlated to T and M stage. Up regulation of miR-615-3p inhibited the apoptosis, promoted proliferation and migration and led to the down-regulation of CELF2. Meanwhile, down-regulation of miR-615-3p resulted in anti-tumor effects. Immunochemistry staining of CELF2 showed its association with T, N and M stage. In addition, overexpression of CELF2 could reverse miR-615-3p's oncogenic functions stated before. These findings indicate that miR-615-3p promotes gastric cancer proliferation and migration by suppressing CELF2 expression for the first time, providing clues for future clinical practices.
Keywords: CELF2; Cell function; Clinical characteristics; Gastric cancer; Gene chips; MiR-615-3p.
参考链接：https://pubpeer.com/publications/8175C7695FD1BEF63736D79DA0E752
本文为【丰倍SCI】公众号原创文章
未经授权禁止转载！
转载请勿更改原文内容及格式！
如有转载需求或合作事宜
可添加下方客服微信
或后台私信小编
三重保障：Al查重＋机械物理查重＋人工查重
官网：www.fengbei-ai.com
客服微信：sindy01010101
