Ann Transl Med
2022 Oct;10(20):1092.
doi: 10.21037/atm-22-1206.
Mesenchymal stem cells alleviate aging in vitro and in vivo
Qun Liu # 1 2, Shaole Song # 1 2, Lei Song 2, Youkun Bi 1 2, Keqi Zhu 1 2, Xinlong Qiao 2, Huiwen Wang 1, Chao Gao 3, Hong Cai 3, Guangju Ji 1 2
Affiliations expand
PMID: 36388801
PMCID: PMC9652517
DOI: 10.21037/atm-22-1206
Abstract
Background: Aging is a natural and multi-factorial phenomenon associated with multiple human pathologies. Mesenchymal stem cells (MSCs) hold great promise in clinical fields of medicine including tissue repair, cardiovascular disease, and brain ischemic injury. The purpose of this study was to explore the roles of MSCs in improving the condition of aging cells, repairing aging tissues and organs, and extending the life span of elderly mice.
Methods: This study was carried out both in vitro and in vivo. We used MSCs to intervene with IMR-90 senescent cells induced by D-galactose and aged C57BL/6 mice.
Results: After 48 hours of co-culturing the aged cells with MSCs, the up-regulated expression of inflammatory factor, interleukin 6 (IL6), and the down-regulated expression of several growth factors, such as transforming growth factor (TGFβ1) and growth differentiation factor (GDF11), in D-galactose induced senescent cells were reversed. Moreover, compared with aged cells, the number of mitochondria and the telomere length were increased with MSC treatment. Similarly, in aged mice, the symptoms related to aging were improved after MSC treatment: the mouse hair became shiny and dense, and the symptoms of bladder overactivity were relieved. Hematoxylin and eosin (H&E) and Masson's trichrome staining showed that the histopathological changes in skin, bladder, liver, and lung were apparently improved.
Conclusions: Treatment with MSCs effectively improves aging-related phenotypes and plays a beneficial role in improving aging and aging-related diseases.
Keywords: IMR-90; Mesenchymal stem cells (MSCs); aging; bladder hyperactivity; cell senescence.
2022 Annals of Translational Medicine. All rights reserved.
参考链接：https://pubpeer.com/publications/A5B7B3E0B77C01B6AC0C356EE657EB
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