2022年3月15日，Biology of Reproduction 在线发表了生殖研究的先锋--Dr. Ryuzo Yanagimachi（点击链接了解Yana更多故事）的最新文章。 Yana现年93岁，在他 60 年科研生涯里一直研究哺乳动物的受精机制。在此综述中，他回顾了哺乳动物受精机制研究了历程，讨论了目前未能解答的话题。
近年来，对受精机制的研究取得了非常多重要进展，伴随着IVF、ICSI等技术的诞生，为增进了人类的福祉做出了重要贡献。但仍有很多待回答的问题，精子如何雌性生殖道运行、穿过卵丘细胞层、透明带，与卵子识别、融合，雌雄原核如何形成和组合等等。现在的实验技术为我们揭开这些谜团提供了重要手段，但需要更先进的成像、基因操纵、生物分子鉴别等手段，来帮助我们更全面的了解受精的机制，最终开发更好的治疗不孕不育或避孕措施。Yana在论文中就以下29个话题进行了讨论，展现了他对这些领域的深刻见解，可以作为相关学者科学研究的重要参考。
1) Transport of rodent spermatozoa through the vagina, uterus and utero- tubal junction
2) Mysteries of the oviduct isthmus
3) Spermchemotaxis
4) Problems of sperm capacitation
5) Sperm hyperactivation
6) Time, site and cause of sperm acrosome reaction in vivo and in vitro
7) Oviductin and oviductosomes: the oviduct's secretory products
8). Size and shape of spermatozoa
9) Roles of protease/proteasomes in sperm capacitation and sperm- oocyte interaction
10) Roles of acrosomal enzymes in fertilization
11) Why is the presence of cumulus oophorus around the egg beneficial for fertilization?
12) How do spermatozoa pass through the zona pellucida of eggs?
13) Is the zona pellucida essential for fertilization and embryo development?
14) The presence of a perivitelline space in unfertilized eggs: A unique feature in mammals.
15) Membrane fusion of sperm and oocyte
16) Sperm-born oocyte activating factor (SOAF): It could be spermatid histone
17) Polyspermy block, with a note on human diploid-triploid mosaics
18) Sperm centrosome and embryo development
19) Fertile life of human oocytes and spermatozoa in oviduct
20) Effect of light on eggs and embryos
21) Puzzles of seminal plasma and sperm competition
22) Similarity between sperm and neurons
23) Intracytoplasmic sperm injection (ICSI): its short history and challenges to be considered
24) Fertilization by round spermatids and spermatocytes
25) Sperm sexing
26) Conversion of somatic cells to germ cells - Artificial gametes
27) Transfer and exchange of sperm chromosomes between two individuals
28) Life without males
29) Human and organ cloning
在致谢部分，Yana表达了对张明觉在内的同事和朋友的感激之情。First of all, I would like to express my sincere gratitude to my mentor Dr. Min Chueh Chang (1908-1991) (Worcester Foundation for Experimental Biology, Shrewsbury, MA) who allowed me to enter this field of investigation......
