使用 TIMS（捕获离子迁移谱 ） 和 4D-Omics （4D-组学）探索复杂代谢组
Exploring the Complex Metabolome with TIMS and 4D-Omics
探讨了 TIMS-TOF 技术在植物科学研究中的代谢组学和脂质组学应用。捕获离子迁移谱 (TIMS) 与小分子研究的结合，在表征生物样品方面具有显著优势，特别是在同分异构体和同分异构体化合物的分离方面。此外，还重点介绍了一种新颖的质量控制功能 RealTime QC，它是一种在获取数据时实时监测 LC、MS 和 TIMS 参数的有效方法。展示了包括 MetaboScape 在内的数据分析工具，用于注释天然产物代谢物和脂质，以及用于鉴定异构体物种的方法。
Applications of TIMS- TOF technology in metabolomics and lipidomics for plant science research were explored. The integration of trapped ion mobility spectrometry (TIMS) into small molecule research offers significant advantages in characterizing biological samples,particularly in the separation of isobaric and isomeric compounds. Additionally, RealTime QC, a novel quality control feature, were highlighted as an efficient method for monitoring LC, MS, and TIMS parameters in real time as data are being acquired. Data analysis tools including
MetaboScapewere demonstrated for the annotation of natural products metabolites and lipids, as well as methods for isomeric species identification.
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