Mol Ther Nucleic Acids
2019 Sep 6:17:455-464.
doi: 10.1016/j.omtn.2019.04.030. Epub 2019 Jun 7.
lncRNA SPRY4-IT1 Regulates Cell Proliferation and Migration by Sponging miR-101-3p and Regulating AMPK Expression in Gastric Cancer
Shuguang Cao 1, Limiao Lin 1, Xuanping Xia 1, Hao Wu 2
Affiliations expand
PMID: 31330497
PMCID: PMC6646863
DOI: 10.1016/j.omtn.2019.04.030
Retraction in
Retraction Notice to: lncRNA SPRY4-IT1 Regulates Cell Proliferation and Migration by Sponging miR-101-3p and Regulating AMPK Expression in Gastric Cancer.
Cao S, Lin L, Xia X, Wu H.Mol Ther Nucleic Acids. 2024 May 19;35(2):102212. doi: 10.1016/j.omtn.2024.102212. eCollection 2024 Jun 11.PMID: 38784177 Free PMC article.
Abstract
Increasing evidence indicates that long noncoding RNA SPRY4 intronic transcript 1 (lncRNA SPRY4-IT1) has been reported to be associated with the progression of several cancers, but its expression level and the function of SPRY4-IT1 in the progression of gastric cancer (GC) have been rarely reported. Here we found that SPRY4-IT1 was upregulated in GC. In vitro experiments revealed that SPRY4-IT1 knockdown significantly inhibited GC cell proliferation by causing G1 arrest and promoting apoptosis, whereas SPRY4-IT1 overexpression promoted cell growth. Further functional assays indicated that SPRY4-IT1 overexpression significantly promoted cell migration and invasion. Bioinformatics analysis predicted that there is a SPRY4-IT1/miR-101-3p/AMPK axis in GC progression. A dual-luciferase reporter system validated the direct interaction of SPRY4-IT1, miR-101-3p, and AMPK. Western blot verified that the inhibition of SPRY4-IT1 decreased AMPK expression. Furthermore, silencing SPRY4-IT1 suppressed GC growth in vivo. Importantly, we demonstrated that SPRY4-IT1 was upregulated in serum exosomes from GC patients and correlated with cancer metastasis. Altogether, silencing SPRY4-IT1 suppresses the progression of GC by interacting with miR-101-3p and decreasing inhibiting AMPK expression. Taken together, our study demonstrates that SPRY4-IT1 could act as a potential therapeutic target for GC patients.
Keywords: SPRY4-IT1; ceRNA; gastric cancer; lncRNA; metastasis; therapeutic target.
参考链接：
https://pubpeer.com/publications/F8B0863E77BED4CE8D6CE10197F484
本文为【丰倍SCI】公众号原创文章
未经授权禁止转载！
转载请勿更改原文内容及格式！
如有转载需求或合作事宜
可添加下方客服微信
或后台私信小编
三重保障：Al查重＋机械物理查重＋人工查重
官网：www.fengbei-ai.com
客服微信：sindy01010101
