在开花植物中,受精作用一旦完成,合子基因组会迅速被激活。
然而,直至目前,我们对于驱动胚胎发育起始的分子途径的认识仍然相当有限。
这篇研究发现,胚胎起始是由受精卵细胞中父本基因组表达的转录因子的协同作用促进的。
以下是文章摘要
中文为机翻仅供参考,以原文为准
Title
Synergistic induction of fertilization-independent embryogenesis in rice egg cells by paternal-genome-expressed transcription factors
题目
通过父系基因组表达的转录因子协同诱导水稻卵细胞进行不依赖受精的胚胎发生
第一作者
Hui Ren
通讯作者
Venkatesan Sundaresan
通讯单位
University of California
摘要
In flowering plants, rapid activation of the zygotic genome occurs after fertilization, but there is limited knowledge of the molecular pathways underlying embryo initiation.
在开花植物中,受精后合子基因组的快速激活是一个关键过程,然而关于胚胎起始背后的分子路径,我们的知识还相当有限。
In rice, a key role is played by the transcription factor BABY BOOM 1 (OsBBM1), initially expressed from the paternal genome.
在水稻中,转录因子BABY BOOM 1 (OsBBM1)扮演着关键角色,它最初从父本基因组表达。
Ectopic OsBBM1 expression in the egg cell can override the fertilization requirement, giving rise to parthenogenetic progeny.
OsBBM1在卵细胞中的异位表达可以绕过受精的需求,从而产生单性生殖后代。
Here we show that the WOX-family transcription factor DWARF TILLER1 (OsDWT1)/WUSCHEL-LIKE HOMEODOMAIN 9 (OsWOX9A), another gene paternally expressed in zygotes, is a strong enhancer of embryo initiation by OsBBM1.
我们的研究显示,WOX家族的转录因子DWARF TILLER1 (OsDWT1)/WUSCHEL-LIKE HOMEODOMAIN 9 (OsWOX9A),另一个在合子中由父本基因组表达的基因,是OsBBM1促进胚胎起始的强大增强剂。
Co-expression of OsWOX9A and OsBBM1 in egg cells results in 86–91% parthenogenesis, representing 4- to 15-fold increases over OsBBM1 alone.
OsWOX9A和OsBBM1在卵细胞中的共表达导致86-91%的单性生殖率,这比单独OsBBM1增加了4到15倍。
These results suggest that embryo initiation is promoted by the synergistic action of paternal-genome-expressed transcription factors in the fertilized egg cell.
这些结果表明,受精卵细胞中来自父本基因组表达的转录因子的协同作用促进了胚胎起始。
These findings can be utilized for the efficient production of haploids, as well as clonal hybrid seeds in crop plants.
这些发现可以被用于高效生产单倍体以及作物中的克隆杂交种子。