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近日，南京工业大学Zhenlei Liu(第一作者)、朱政明副教授(通讯作者)和江凌教授(通讯作者)等在国际期刊《Journal of Agricultural and Food Chemistry》发表了题为“Clostridium tyrobutyricum in Combination with Chito-oligosaccharides Modulate Inflammation and Gut Microbiota for Inflammatory Bowel Disease Treatment”的封面文章。
结果表明，合生元补充剂有效地缓解了炎症并恢复了肠道屏障功能。此外，合生元补充剂可有助于消除活性氧（ROS），并通过酪丁酸梭菌的短链脂肪酸产生菌提高短链脂肪酸的产生。而且，这种合生元还可以调节肠道微生物的组成。这些发现强调了酪丁酸梭菌和壳寡糖作为有价值的活体生物治疗药物在治疗肠道相关疾病方面的潜力。
图形摘要
Figure 1. Principle of probiotics in the treatment of intestinal diseases and experimental design in mice. (A) The study involved three treatment groups: the C.t. group received oral administration of C. tyrobutyricum, the COS group received oral administration of COSs, and the synbiotic group received oral administration of both C. tyrobutyricum and COSs; (B) colitis was induced in mice by adding 2.5% DSS to their drinking water for 7 days. Daily oral gavage with C. tyrobutyricum, COSs, or the synbiotic commenced on the sixth day of induction. Ctrl: healthy control group fed a normal diet.
Figure 2. Macroscopic assessment of the therapeutic effects of C. tyrobutyricum, COSs, and the synbiotic on DSS-Induced colitis in mice. (A) Changes in body weight; (B) DAI; (C) macroscopic images of the mice colon and (D) corresponding quantitative colon length; (E) spleen weight-to-body weight ratios.
Figure 3. Assessment of damage and therapeutic efficacy of C. tyrobutyricum, COSs, and the synbiotic in DSS-Induced colitis in mice. (A) Microscopic images of proximal colon tissue stained with H&E; (B) Histopathologic scores, calculated after colon microscopy analysis; (C) MPO activity in proximal colon tissue.
Figure 4. Impact of C. tyrobutyricum, COSs, and the synbiotic on the modulation of immune markers in DSS-treatment mice colon. Levels of cytokine proteins, including (A) IL-1β, (B) TNF-α, (C) IL-6, (D) IL-10, (E) TGF-β, and (F) ROS, were analyzed by ELISA in proximal colonic tissues.
Figure 5. Impact of C. tyrobutyricum, COSs, and the synbiotic on tight junction protein expression in DSS-Induced mice colon. (A) Microscopic images of proximal colon tissues immunohistochemically stained with occludin and ZO-1. Scale bar represents 100 μm. Arrows indicate IHC positivity; (B) the average optical density of each group is depicted.
Figure 6. Concentrations of acetate (A), propionate (B), and butyrate (C) in feces.
Figure 7. Regulation of gut microbiota composition by C. tyrobutyricum, COSs, and the synbiotic. (A–C) Alpha diversity indices; (D) OUT cluster analysis; (E, F) relative abundance of fecal bacterial phylum and family; (G) variations in microbial taxa among different groups analyzed by LEfSe.
https://doi.org/10.1021/acs.jafc.4c03486
江凌，1982年生，中共党员，南京工业大学学科处副处长、教授，博士生导师，国家“优青”获得者，国家一流本科专业负责人、江苏省杰青、江苏省重点学科带头人，黄和院士团队核心成员，新疆“天池英才”柔性引进人才，新疆农业科学院微生物应用研究所特聘教授，新疆特殊环境微生物实验室学术委员会委员。
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肉与肉制品 蛋与蛋制品 水产品 奶及奶制品
豆及豆制品 果蔬及果蔬制品 大米及米制品 食用菌
炎症性肠病 糖尿病 肝病 神经疾病
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