内容简介
本研究论文聚焦基于离子液体的透明膜耦合人体肺上皮芯片在呼吸机械应力下揭示PM0.5污染效应。近年来，人类暴露于不同尺寸颗粒物（PM）的可能性不断增加。由于粉尘传输对气候和天气的影响，已在大气中检测到不同大小的颗粒物。肺上皮细胞作为调节器，负责协调对局部损伤的先天性免疫反应。本研究开发了一种肺上皮芯片平台，由易于模塑的聚二甲基硅氧烷（PDMS）层与一层薄而灵活的透明离子液体基聚（羟乙基）甲基丙烯酸酯凝胶膜组成。通过在该膜上培养人肺上皮细胞（Calu-3），形成了肺上皮细胞层。采用基于Arduino的伺服电机系统在吸气/呼气期间于气-液界面施加单轴拉伸力（10%应变，0.2 Hz频率），从而模拟机械应力。随后，在静态、动态和动态+机械应力（DMS）条件下向该芯片平台加入平均粒径为463 nm的二氧化硅纳米颗粒（PM0.5），以研究环境污染物对肺上皮的影响。通过测定乳酸脱氢酶释放及肿瘤坏死因子-α（TNF-α）的量化分析促炎反应，结果显示出上皮细胞的变化。
引用本文（点击最下方阅读原文可下载PDF）
Kaya B, Yesil-Celiktas O, 2024. Ionic liquid-based transparent membrane-coupled human lung epithelium-on-a-chip demonstrating PM0.5 pollution effect under breathing mechanostress. Bio-des Manuf 7(5):624–636. https://doi.org/10.1007/s42242-024-00289-z
文章导读
图1 图片摘要
图2 离子液体基聚（羟乙基）甲基丙烯酸酯膜的合成及表征
图3 肺上皮的形成
图4 PM0.5对肺上皮芯片的影响
参考文献
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